????? ??????????? ????? Column Chromatography

1
??? 6

???????????
???? 1
??? 6
2
??? ????????...

• ??? ????? ???? - ???? ??????, ???? ???????????
  ...
• ??? ??? ???? ?????, ??????

???? 2
??? 6
3
??? ????? ?????????

• ???? ????? ??????????? ?????
• ????? ??????
• ???? ???? ?? ???? ???????????
• ?????? ?????
• ????? ???????????

???? 3
??? 6
4
????? ??????????? ?????Column Chromatography
???? ????? Stationary Phase
???? 4
??? 6
5
?????

???? 5
??? 6
6
?? ???? ??????? ?????? ??? ?????? ??????

???? 6
??? 6
7
????? ???????????
???? 7
??? 6
8
???? ?? ????

???? ???? ????? ?? ??? t
???? 8
??? 6
9
???? ????? ????? ?????

(6.3)
???? ????? - ???????? Rs ????? ??????? Rsgt1.6
???? 9
??? 6
10
???? ?????? ???????????

??????? ?????? ?????? ??????...
??? ???????????
??????
?? ???????? Gel Filtration Gel Permeation
Chromatography, GPC Size Exclusion
Chromatography, SEC
???? ????????
???????, ????? ?????
???? 10
??? 6
11
???? ?????? ??????????? (????)
??????? ?????? ?????? ??????...
??? ???????????
??????
????? Adsorption Chromatography
??????????? ??-????????
???????, DNA, RNA
???? 11
??? 6
12
????? ?????Packing Materials

?? (GPC)
???? 12
??? 6
13
?? - ????????
?? ???????? Gel Filtration Gel Permeation
Chromatography, GPC Size Exclusion
Chromatography, SEC
??? 6
???? 13
14
??????????? ?????????????? ?????
????? ??????????? ??????????? ???-????????
???????????? ???????????

• ??????????? ?????
• ?????? butyl ?? phenyl ?????? ???? ????
• ?????? ????, ????? ??? ????

??????????? ?????????? Hydrophobic Interaction
Chromatography, HIC
??? 6
???? 14
15
????? ?????Ion-Exchange Chromatography
????? ??????????? ??????? ???????, ?????? ?????,
?????? ????????
????? (????) ?? ????? ?????? ?????? ???? ???? ???
?????? ??????
????? ?????? Counter ions
????? ???????
????? ?????? Fixed ions
??? 6
???? 15
16
??? ???? ?????? ??????...

• ?????? ????? ???? (Gradient methods)
• ???? ????? - ????? ??????? ?? ????? ???
• ???????????? ??????????? - ????? ??????
  ??????? ???????

• ????? ?????? (Displacement chromatography)
• dextran sulfate
• carboxymethyl strach
• imminodiacetic acid (IDA)

??? 5
???? 16
17
??????????? ????? ??????? Radial flow
chromatography
??? 5
???? 17
18
????? ???????

• ???????
• ????? ??? ???
• ????? ????? ?????
• ?????? ?????
• ???????? ??????

????? ?????? ???? ???? ???? (?????? ?????? ?????
????)
??? 5
???? 18
19
?????? ??????????? ????? ???????
??? ????? ????? ????? ( ???? ????? ????? ?????
???? ???????) ????? ???? ?????
?????? ??????!
????? ??? ?????, ??? ??????, ?????? ???? ???????
????? ????? - ??? ????? ???? ??????
??? 5
???? 19
20
??????????? ?????
Annular Chromatography
??? ????? ?? ??? ??????
??? 5
???? 20
21
????? ??????????? ?????????
????? ???????
??? 5
???? 21
22
????? ??? ?????

• ???? ?????? ??????????? ?????
• ????? ??????
• ?????
• ????? ????? ?????
• ???? ????? ?? ??????????? ????? ????????
• ???? ?????? ???? ?????, ???? ????? ?????

??? 6
???? 22