Lentikats Biotechnology in wastewater treatment

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Lentikats Biotechnologyin wastewater treatment
Biotechnologie, které mení svet...
Nitrogen pollution specialists
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Content

• Our company
• What is Lentikats Biocatalyst?
• Advantages of Lentikats Biotechnology
• Comparison of Lentikats Biotechnology with
  traditional processes
• Key benefits of Lentikats Biotechnology
• Application of Lentikats Biotechnology
• Partners
• References
• What can we offer?
• Contacts

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Lentikats Biotechnology inwastewater treatment
plants

• The LentiKats corporation continues a nine year
  old tradition of the research and development of
  a new leading edge technology to be integrated
  into the wastewater treatment industry.
• Lentikats Biotechnology has demonstrated success
  in the elimination of both high and low
  concentrations of nitrogen in wastewater with the
  efficiency of 98 and higher.
• We specialize in the efficient elimination of
  nitrogen pollution.
• We are flexible and competent in preparing custom
  made solutions.

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What is a Lentikats Biocatalyst?

• Unique shaped of the matrix and the original
  principle of immobilisation enable the
  encapsulation of free enzymes or microorganisms
  (bacteria, yeasts, fungi) in a stable porous PVA
  (polyvinylalcohol) carrier. The carrier
  advantages are
• Excellent physio-mechanical characteristics that
  provide for long-term mechanical stability,
• Low biodegradability,
• Zero toxicity.
• Lentikats Biocatalyst enables easy separation of
  enzymes and microorganisms from the surrounding
  media and therefore it can be reused multiple
  times.

Biotechnologies are changing the world
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How does Lentikats Biocatalyst function?

• Due to its maximum surface and at the same time
  minimum volume of the Lentikats Biocatalyst, the
  diffusion limitations associated with the
  transport of the substrate into the lens and the
  product out of the lens are significantly reduced.

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Nitrification

• Ammonia nitrogen is oxidised to nitrites by
  bacteria Nitrosomonas
• (N. europaea, N. oligocarogenes, ) or
  Nitrosococcus, Nitrosospira and Nitrosolobus.
• NH4 1,5 O2 ? NO2- 2 H H2O
  
• Nitrite is oxidised to nitrates by bacteria
  Nitrobacter (N. agilis, N. winogradskiy) or
  Nitrospira and Nitrococcus.
• NO2- 0,5 O2 ? NO3-

Nitrification summary NH3 2 O2 ? NO3-
H H2O
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Denitrification

• Denitrification one-step biochemical process
• Denitrifying bacteria (Paraccocus sp.) utilises
  nitrate nitrogen as electron acceptor instead of
  oxygen to create gaseous nitrogen
• Ared NO3- ? N2 Aox
  energy

Denitrification summary Ared NO3- ?
N2 Aox energy
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Lentikats Biotechnology lay-out
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Your demands?

• to meet effluent limit concentration of nitrogen,
  
• to intensify your WWTP without extensive
  reconstruction,
• to reduce operational and investment costs when
  building a new WWTP,
• to enhance nitrification and denitrification
  process,
• to become more environmentally oriented (reduced
  electricity consumption, lower waste production,
  lower sludge production),
• to simply become better and more efficient
  (reduced costs).

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Comparison of Lentikats Biotechnology with
conventional wastewater treatment technologies

• Reduction of nitrification and denitrification
  tank volume, secondary settlement tank
  elimination.
• Lower electricity consumption, lower organic
  matter demand, lower oxygen demand in
  nitrification.
• Lower waste and sludge production, reduction of
  sludge storage tank volume.
• High process stability.
• Ability to respond to even highly fluctuating
  concentration.

Want to be more efficient? Want to be more
environmentally concerned? LentiKats a.s. is the
right business partner for you.
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Lentikats Biotechnology benefits 1. reaction
volume reduction

• Higher concentration of nitrification and
  denitrification organisms
• Higher reaction rate - up to 500-800 mg N (NH4,
  NO3-) per hour and kg of Lentikats Biocatalyst.
• Shorter retention time in nitrification (12-50
  hr) and denitrification (3-6 hr).
• Reduction of reaction volumes.

Activated sludge 5 nitrifying microorganisms
BL 100 nitrifying microorganisms
X
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Lentikats Biotechnology benefits 2. lower
operational material demand
Activated sludge
Lentikats Biotechnology

• Oxygen and organic substrate is utilised only for
  the biochemical conversion, not for production of
  new biomass.
• Electricity and heat consumption reduction due to
  smaller reaction tanks.

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Lentikats Biotechnology benefits 3. lower sludge
production
Activated sludge system
Lentikats Biotechnology
AS
ST
microsieve
BL
Sludge management

• Bacteria is immobilised inside the Biocatalyst,
  i.e. there is no need for further production of
  new biomass, actual biomass production is very
  limited (2 g/m3 where BOD lt 50 mg/l).
• Sludge production is up to 170 times lower in
  industrial wastewater with low concentration of
  organic matter.

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Lentikats Biotechnology benefits 4. high process
stability

• Porous outer shell protects microorganisms while
  creating inside micro-climate higher
  robustness towards chemical shocks.
• High concentration of microorganisms high
  working concentration (up to 500-800 mg/l N-NH4,
  10 g/l N-NO3-)

• Long lifetime 1.5 to 3 years guarantee.

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Lentikats Biotechnology benefits 5. ability to
respond to extensive fluctuations

• It is possible to set-up and operate the process
  with high precision and efficiency of 98 and
  higher due to the ability to dose accurate amount
  of Biocatalyst.
• Maximal system capacity defined by amount of
  Biocatalyst in reactor with activity
  corresponding to optimal conditions.
• Real system performance is possible to
  effectively manage by regulation of air/organic
  substrate dosage.

Biotechnologies are changing the world
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What do you gain?

• Costs saving
• no financial sanctions,
• operational costs saving (lower organic substrate
  demand, reduction of costs for sludge management
  and disposal),
• investment costs saving (intensify existing WWTP
  without extensive reconstruction while building
  large tanks).
• 2. Smaller footprint (reduction of reaction
  volumes).
• 3. You are more environmentally concerned (lower
  energy consumption, reduced waste production).
• Sludge management reduction (up to 170 times
  lower sludge production).
• Reduced operational risks (controlled process,
  high performance under low temperature, high
  process stability).
• In case of industrial wastewater with low
  organic matter content..

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Field of application

• Municipal wastewater
• Intensification of existing wastewater treatment
  plants without extensive reconstruction to
  decrease effluent concentration of nitrogen.
• Industrial wastewater
• Removal of high concentration of nitrogen
  compounds (N-NH4,,N-NO3-, ,N-NO2- ) and hardly
  degradable organic compounds.
• Biogas plants
• Removal of nitrogen from reject water prior to
  its discharge into recipient/WWTP.

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1. Intensification of municipal WWTP by removal
of nitrogen from sludge water - scheme
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1. Intensification of municipal WWTP by removal
of nitrogen from sludge water

• System designed to effectively remove nitrogen
  from sludge water, which can make up to 10-20
  of total mass of nitrogen entering biological
  unit in conventional municipal WWTPs.
• Multiple reduction of investment costs in
  comparison to traditional ways of WWTPs
  intensification substantially smaller reactors.
• Applicable for WWTPs with extensive sludge
  management (stabilised sludge dewatering).
• Limited application by actual content of nitrogen
  in sludge water.

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2. Intensification of municipal WWTP by removal
of residual nitrogen from effluent stream - scheme
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2. Intensification of municipal WWTP by removal
of residual nitrogen from effluent stream

• Applicable for any WWTP, which need to meet
  effluent criteria
• Mostly reduced to denitrification only in case of
  conventional municipal WWTPs.
• Multiple reduction of investment costs in
  comparison to traditional ways of WWTPs
  intensification substantially smaller reactors.
• System works slower at colder temperatures, yet
  the lifetime of Lentikats biocatalyst then
  extends (2 to 3 years).
• It is possible to set up the system for exact
  amount of nitrogen to be removed and further
  effectively manage the efficiency by simple
  correction of the amount of Biocatalyst in the
  system.

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3. Removal of high and low concentration of
nitrogen from industrial wastewater

• Simple, compact and highly efficient system.
• Eliminates the demand of organic matter in
  nitrification for evolution of new biomass (in
  contrast to systems utilising suspended biomass).
  
• Substantial reduction of operational costs in
  comparison to traditional technologies.
• Possibility to set up the system to precisely
  defined requirement.
• Enhanced resistance towards environmental effects
  (fluctuating concentration of nitrogen, pH,
  presence of toxic compounds, etc.).
• High system stability, easy system operation.

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4. Nitrogen removal from wastewater with highly
fluctuating nitrogen concentration (industry,
hotels, petrol stations, etc.)

• Maximal capacity is defined by total amount of
  Lentikats Biocatalyst.
• Actual performance is effectively managed by
  regulation of air/organic substrate dosage.
• Easy process automation.
• High process stability
• Substantial reduction of operational and
  investment costs.

Biotechnologies change the world.
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5. Nitrogen removal from wastewater with seasonal
exceeding of effluent limits

• Possibility to utilise Lentikats Biocatalyst in
  its full capacity only temporarily and modify the
  performance or store it away during off-season
  period.
• Wide utilisation and operation flexibility.

Biotechnologies change the world.
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6. Nitrogen removal from reject water in biogas
plants

• Lentikats Biotechnology allows for working
  concentration of N-NH4 of up to 800 mg/l, which
  is almost 4 times higher than what activated
  sludge system allows.
• Removal efficiency of 98 and higher with
  flexible regulation.
• Applicable for pre-treatment of reject water
  prior to its discharge to recipient/WWTP.
• Pure oxygen application in nitrification
  alternative.
• Applicable also as a tertiary step after previous
  treatment.

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7. Special application of Lentikats Biotechnology
in wastewater treatment

• Solutions from ion exchange regeneration
• high concentration of nitrate nitrogen (up to 2,5
  g/l N-NO3-),
• high concentration of inorganic salts (up to 20
  g/l NaCl),
• enables repetitive use of regeneration solution.
• Selective biodegradation
• selection, cultivation and immobilisation of
  suitable microbial cultures to remove hardly
  degradable organic compounds.

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Lentikats Biotechnology Applications

• Lentikats Biocatalysts and applied Lentikats
  Biotechnology also delivers additional value and
  benefits in the following sectors
• distillery production of bioethanol,
• pharmaceutics production of robust
  biocatalysts,
• food industry whey processing, production of
  special sugars carbohydrates and
  syrups.

Biotechnologies change the world
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Our partners in the field of industrial
applications
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Our partners in the field of research and
development
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Our references and experience

• Pilot continual unit for nitrification and
  denitrification of industrial waste water
  (rendering plant, meat-packing, chemical
  industry, pharmaceutical industry) (Chemopetrol
  a.s., ASAP Ve u Havlíckova Brodu, Biotica Inc.
  ICN Roztoky u Prahy etc.)
• Pilot semicontinual unit for nitrification
• and denitrification in WWTP Svitavy
• Pilot semicontinual unit for nitrification
• and denitrification in WWTP Ceská Lípa

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Why Lentikats Biotechnology?

• It presents a highly efficient, cost-effective
  and low-operation demand method for removal of
  nitrogen from wastewater.
• It is the only economically viable solution for
  removal of high concentration of nitrogen.
• Risk minimisation on investors side. We offer
  financial participation on the pilot-scale test
  phase of your project. You shall decide about the
  project execution after the testing period.
• We guarantee designed and approved effluent
  parameters.

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What can we offer to you?

• Have we raised your interest in testing Lentikats
  Biotechnology in your own project? We are
  prepared to supply a sufficient amount of our
  Biocatalyst and provide full assistance in order
  to convince you of the obvious advantages our
  technology has when applied in your treatment or
  biogas plant.
• For all companies introducing new technologies,
  the first applications and results are the most
  important ones. That is why we offer excellent
  conditions to potential clients in an effort to
  eliminate any risks.

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Lentikats Biotechnology service

• Analysis of water influent and effluent
  parameters.
• Control of maintenance of specified technological
  procedures and conditions.
• Periodical monitoring of Lentikats Biocatalysts
  activity.
• Supply and replacement of Lentikats Biocatalyst
  in such periods and volumes to sustain the
  required Lentikats biotechnology parameters.
• Disposal of the spent biocatalyst.

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Contacts

• Companys Headquarters
• LentiKats a.s.
• Evropská 423/178
• 160 00 Praha 6
• Czech republic
• Tel. [phone]
• Fax [phone]
• E-mail info_at_lentikats.eu
• Production plant and laboratories
• LentiKats a.s.
• Pod Vinicí 83
• 471 27 Strá pod Ralskem
• Czech republic
• Tel. [phone]