Figure 1. Melting curve of SNP marker D1 of probe and PCR amplicon on LightScanner. SNA D1 is

1
Figure 1A
Figure 1B
Figure 1. Melting curve of SNP marker D1 of probe
and PCR amplicon on LightScanner. SNA D1 is CA
or CA deletion. It is very clear to see the
genotype by the melting curve of unlabeled probe
and the melting curve of amplicon also could
distance the genotype too. So that the genotype
could confirm by both way. A) Melting curve of
unlabeled probe. B) Melting curve of amplicon.
2
A
G
G
A
A G
Figure 2A
Figure 2B
Figure 2. Melting curve of SNP marker M1 of probe
and PCR amplicon on LightScanner(n16). SNA M1
is A or G. It is very clear to see the genotype
by the melting curve of unlabeled probe and the
melting curve of amplicon also could distance the
genotype too. So that the genotype could confirm
by both way. Figure2A. Melting curve of
unlabeled probe. Figure2B. Melting curve of
amplicon.
3
GT GC
GT GC
Figure 3B
Figure 3A
Figure 3. Human genomic wild type DNA (CFTR 542
G) and cystic fibrosis mutant DNA (CFTR 542 T)
were mixed in different ratios. As mutation
mixed with wild type increase, the GC peak
highs are decreased and GT peak highs are
increased. A) 10 increments of mutation from 0
to 100. B) 2 increments of mutation from 0
to 10.