Lyophilization

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Lyophilization
318 711 Dr. Thanaset Senawong
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Lyophilization

• Lyophilization ???? Freeze-drying ???
  ??????????????????????????????????????????????????
  ??????????????????????????????

? Lyophilization (lyophilic solvent
loving)
? Lyophilized
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??????????????????? Freeze-drying
- ??????????????? ???????? ???????????
??????????????????????????????

• ???????????? ???????????????????????????????????
  ????????????

- ???????????????? ???????????? ?????????
???????????????????
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???????????Lyophilization
- ????????????????????????????????????????????????
????????????????
- ????????????????????????
- ?????????????????????????????? ???????????????
- ????????????????????????????????????????????????
?????
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??????????Lyophilization
? ???????????????????? ?????????
? ??????? lyophilized ?????????? ??????????
???????
? ???????????? ??????????
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???????????? Lyophilized ??????

• microbial culture
• ????????
• ?????
• ?????????????
• ??
• ??????
• ????????
• ?????????
• ?????????????????

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???????????? Lyophilized

• Freezing ????????????????????????????????????
• ????????????????????????????
  ????????
• ??????????????? (ice)

• Vacuum ?????????????????? (sublimation) ????
• ????????? (water vapor)
  ????????
• ?????????

• Heat ????????????????????????????????????????????
  ?
• ???? ??????????????????????????????
  ?
• (eutectic temp) ???????????????????

4. Condensation ????????????????????????????????
?????????????????????????????
??????? ??????? (low-temp
condenser plate)
?????????????????????????
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• ????????????????????????????????????
• Pre-freezing

• ????????????????????????????????????????????
• ?? 2 ???????

1. Primary drying ?????????? ?????????????
frozen sample ???????? 2. Secondary drying
???????????????????????????? solid material ??
sample ???????? ??????? drying sample ???????????
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• Drying Time ???????
• vapor pressure ?????????? condensor temperature
• ?????????????????????????????????? ? ???????????
• ?????????????? ??? ???????????????????????
• condensor temp.

??????? eutectic temp ?????????????????????
freeze drying ---gt??????? biological material
???? ???????? ????????? 0?C ?????? -40
?C ????????
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?????????????????????????????????? ?
????????
?????? -10oC, ????????? 1.95 mm.
Condensor ??? -50oC, ???? ????? 0.0269 mm.
????????? ?????????? 1.923 mm.
??????????? ??????? lyophilization
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• ?????????????????????????? product ?????? ?
  ?????? product ????
• Secondary drying ???? final phase ???
  lyophilization
• - ?????? bound ??? solid material ?? sample
  (???????? sorbed water) ?????????????????? ?????
  desorption process
• - Process ?????? ????? sorbed water ????????? lt
  liquid ???????????????????
• Freeze-drying ?????????????????????????? sample
  ??? condensor ???????
• ??? remove ????????????? ????????????????????????
  ??????

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????????????? Freeze-Drying System
1. Chamber ???? Manifold ?? pre-frozen sample 2.
Vacuum System ?????????????????? flow ????? 3.
Heat Source ???????????????????????? 4. Low
Temperature Condensor ????????????????????????????
????????????? ?????? (trap) ??????????????????
vacuum pump
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Basic Industrial Freeze Dryer
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Schematic of a Laboratory Freeze Dryer with
Shell Frozen Flasks Attached to a Manifold
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??????????????? Freeze-Dryer
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Shell Freezer
Freeze Dryer
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Accessory Part
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Accessory Part