Sampling and Analysis of Pesticides from Barrier Treatments

1
Sampling and Analysis of Pesticides from Barrier
Treatments

• Brian P. Quinn, Ulrich R. Bernier, Daniel L.
  Kline
• USDA-ARS-CMAVE-MFRU

2
Climate

• Hot and dry Iraqi theater
• Wetlands Rice fields
• Tropics
• Temperate areas

3
Vegetation

• Vegetation type
• Density
• Plant integument

4
Mosquito Species

• Define the species composition that requires
  control
• Gain an understanding of the resting behavior of
  each species of concern

5
(No Transcript)
6
Vegetation Sampling

• Inert sample containers are imperative
• In the absence of specially constructed bags,
  glass containers are the most inert choice
• Sampling apparatus must be cleaned regularly

7
(No Transcript)
8
Vegetation Sampling

• Sample size is critical to reproducible results
• The ideal minimum sample is 1-2 kg
• IR4 program that registers pesticides on
  minor-use commodities requires 4 kg minimum
  samples

9
Vegetation Sampling

• Grab sampling A localized way of sampling that
  gives very limited spatial data
• Example Picking one spot in your barrier and
  collecting a sample from only that one location

10
Vegetation Sampling

• Representative sampling Taking vegetation from
  the entire barrier or a defined larger section to
  better represent the average pesticide
  concentration in that area
• Example Sampling vegetation high and low, back
  to front, and side to side.

11
(No Transcript)
12
(No Transcript)
13
(No Transcript)
14
Mass Spectrum of Bifenthrin
15
Chromatogram of Treated Vegetation
Bifenthrin
16
Backpack Fogger vs. Electrostatic Sprayer (0.11
lb acre Talstar)
17
Electrostatic Half-Label Rate vs. Full-Label Rate
18
Barrier Treatment Projects

• Arkansas Rice Fields
• Camp Blanding
• Cage Studies in Gainesville
• Plant Integument Studies in Gainesville
• St. Augustine, FL
• Coachella Valley, CA

19
Summary

• Chemical analysis of barrier treatments is
  critical to understanding efficacy over time in
  differing climates and vegetation types
• Pairing these data with field and laboratory
  toxicity assays provides a most useful tool for
  determining choices of pesticides and treatment
  intervals

20
Acknowledgements

• DWFP funding
• Jacksonville Naval Air Station NECE
• Clint Hoffmann USDA ARS
• David Dame and Max Meisch
• Rui-De Xue Anastasia MCD
• Natasha Elejalde
• Greg Allen
• Nathan Newlon