Adaptive Immunity: 2 Branches

1
Adaptive Immunity 2 Branches
Humoral
Cell-mediated
2
Antibodies

• Tutorial and Problem Set
• http//www.biology.arizona.edu/IMMUNOLOGY/tutorial
  s/antibody/prob_set.html
• Recombination
• http//www.immuteach.de/

3
Generic Ab
4
Ribbon diagram of a monoclonal ab
5
Variability of residues in different abs. The 3
hypervariable regions are termed
complementarity- determining regions (CDR)
6
Side-view of Angiotensin II bound to Fab.
Van der Waals surface contacts
7
(No Transcript)
8
The Ig superfamily
9
(No Transcript)
10
Organization and Expression of Ig Genes
11
(No Transcript)
12
(human chromosome 22)
(human chromosome 2)
(human chromosome 14)
Gene arrangements
13
From Hozumi and Tonegawa, 1976, PNAS USA 73 3628
14
(No Transcript)
15
Circular DNA isolated from thymocytes undergoing
TCR rearrangement (these rearrange just like Ab
genes)
16
(No Transcript)
17
Kappa gene expression
18
H-gene expression
19
Recombination signal sequences
20
(No Transcript)
21
(No Transcript)
22
(No Transcript)
23
(No Transcript)
24
(No Transcript)
25
(No Transcript)
26
(No Transcript)
27
When ss DNA is generated
Extra nucleotides can also be added
Nucleotide addition during joining
28
(No Transcript)
29
Somatic variation
30
Secreted vs. membrane forms of IgM
31
(No Transcript)
32
Class switching by alternative processing
33
Class switching by alternative processing
34
(No Transcript)
35
(No Transcript)
36
Secretory pathway
37
(No Transcript)
38
cis-acting sites
39
Allelic exclusion (only in B and T cells)
40
Model of allelic exclusion