genscriptprobio (1)

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Methods to Generate Stable Cell Lines
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• There are several options available to
  generate stable cell lines depending on the
  purpose of the experiment.

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Culture Conditions

• Culture conditions (passage, split rhythm,
  number, etc.) Stable cell lines can only be
  created if you have the right cell type.

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Design experiment

• Create an experiment and select the expression
  vector, cell type, and transfection method. Check
  that the expression vector and transfection
  methods you choose are appropriate for your cell
  type.

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Select the G418 concentration and the cell number

• Matrix titration can be used to determine the
  appropriate G418 concentrations and cell numbers
  per well. Attention to the fact that stock G418's
  active concentration can vary from
  batch-to-batch.

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Transfection

• Transfect expression plasmid manufacturing to
  cells. Follow the instructions provided by your
  transfection device manufacturer. G418 should not
  be added to the culture medium right away after

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Cell selection

• Transfect cells with G418 and culture them in
  medium containing the appropriate amount of
  G418. You will get a mixed population of
  drug-resistant cells.

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Analyze

• You should choose the right method to analyze
  your stable-transfected cells.

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Thank You

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