CSIR NET Life Science Important Topics

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CSIR NET Life Science Important topics
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Being a broader subject, life science has many
topics which make the CSIR NET syllabus
huge. So, how you have to deal with all these
topics? Dont worry as here weve provided the
list of important topics of CSIR NET life science
subject. You are suggested to check the complete
presentation..
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Unit -1 Molecules and their Interaction Relevant
to Biology

• The stabilizing bonds between macromolecules
• Protein conformations, dihedral bonds and
  Ramachandran plot, techniques to determine
  different conformations, and their sequencing.
• Metabolic pathways of carbohydrates including
  glycolytic and TCA cycle steps, regulation
  (specially allosteric and feedback inhibition),
  enzymes cofactors involved and stoichiometry, ETC
  and ATP synthesis (along with inhibitors) (very
  important)

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Unit -1 Molecules and their Interaction Relevant
to Biology

• Protein conformations, dihedral bonds and
  Ramachandran plot, techniques to determine
  different conformations, and their sequencing.
• Numerical questions on molarity, pH buffer and
  thermodynamic (delta G calculation in various
  cellular processes).

Get Detailed information for CSIR NET Life
Science from Here.
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Unit -2 Cellular Organisation

• Protein transport pathways to various organelles,
  cytoskeletal components like actin, dynein,
  kinesin and their function in different cellular
  processes, enzymatic contents of cellular
  organelles like Golgi, lysosomes, peroxisomes,
  diseases associated with their impaired
  functions, protein modifications in ER.
• Lipid rafts, FRAP, freeze etching.
• Cellular fractionation-based questions will also
  be asked.

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Unit -2 Cellular Organisation

• Various types of ion channels, pumps, and
  transporters, their inhibitors (its very
  important to memorize their names and exact mode
  of inhibition, membrane destabilizing agents,
  detergent treatments (mostly experimental based
  questions are expected), membrane potential and
  what causes a change in the potential.
• Membrane structure and function The composition
  and nature in detail, all types of phospholipids
  and proteins, their distribution in the membrane,
  RBC membrane components such as Band.

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Unit 3 Fundamental Processes

• RNA Transcription Transcription factors in
  prokaryotes and eukaryotes, RNA polymerase,
  machinery, initiation complex formation, capping,
  polyadenylation, types of RNA splicing
  spliceosome components, RNA editing.
• DNA Replication Meselson Stahl Experiment,
  prokaryotic and eukaryotic replication, different
  types of DNA polymerases and their specific
  functions.

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Unit 3 Fundamental Processes

• Gene regulation in eukaryotes Phage gene
  regulation Lytic and lysogenic phases and genes
  expressed.
• Individual stages of the processes (initiation,
  elongation, termination) and the components
  involved (like enzymes, activators, inhibitors,
  repressors)
• Gene silencing methods, RNAi

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Unit 4 - Cell Communication and Cell Signaling

• The 1st subunit
• The 2nd subunit deals with cell signalling
• The 3rd subunit is about cell communication
• 4th subunit

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Unit 5 Developmental Biology

• Gametogenesis with special emphasis on stages and
  chromosome number, fertilization process, slow
  block and fast block to polyspermy.
• Types of eggs and various cleavage patterns and
  examples of organisms in which it takes place,
  fate maps.
• The important terms and terminologies such as
  potency, commitment, induction, competence,
  cytoplasmic determinants and morphogenetic
  gradients in-depth concepts with reference to
  the different developmental processes.

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Unit 5 Developmental Biology

• Drosophila developmental stages all types of
  maternal and zygotic genes involved with the
  detailed process of function. Very scoring topic
  overall!
• Differentiating between terms such as
  specification, differentiation, and
  determination. Types of specifications such as
  autonomous, conditional and regulative with
  examples.
• From plant development focus on topics such as
  double fertilization, leaf development, meristems
  and their transition to flowering, and complete
  floral development (along with ABC model) with
  emphasis on the genes involved in Arabidopsis and
  Antirrhinum.

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Unit 6 System Psychology - Plant

• Biotic and abiotic stress Plants detailed
  mechanisms for adapting to different stress
  conditions.
• Sensory photobiology phytochrome, cryptochrome,
  phototropin, photoperiodism, short-day plants,
  long-day plants, behavior and response of a plant
  to different colored lights.

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Unit 6 System Psychology - Plant

• Photosynthesis C2, C3, C4, CAM pathway, TCA,
  photorespiration, oxidative phosphorylation,
  cyclic and noncyclic photophosphorylation.
• Solute transport, Water potential concept.
• Stomatal opening and closure by various triggers.

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Unit 7 System Psychology Animal

• Cardiac cycle, heartbeats, blood pressure, blood
  volume regulation, different serum contents.
• Different neurotransmitters and action potential,
  you may expect a combined question with Unit 2
  with respect to membrane or Unit 4 with respect
  to cell signaling.
• Gaseous exchange hemoglobin, myoglobin, their
  behavior with oxygen like left and right shift.

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Unit 7 System Psychology Animal

• Kidney filtration system, structure, and function
  of different parts of a nephron, regulation of
  water balance.
• Sense organs structure and function of
  different parts of the ear.
• Reproduction-hormones in processes such as
  gametogenesis and ovulation.

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Unit 8 Inheritance Biology

• Different genetic crosses, pedigree, ABO blood
  grouping, multiple genes, complementation,
  mitotic and meiotic non-disjunction, tetrad
  analysis
• Mutations (mutational studies based questions
  expected) which can be also covered under Unit 3.
• Microbial genetics and interrupted mating
  experiments, (Hfr mapping, methods of genetic
  transfers transformation, conjugation,
  transduction and sex-duction, mapping genes by
  interrupted mating).

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Unit 8 Inheritance Biology

• Mendelian ratios, their statistics, terms like
  codominance, incomplete dominance, pleiotropy,
  genomic imprinting, penetrance, expressivity,
  phenocopy, linkage, crossing over, sex linkage,
  sex limited and sex influenced characters,
  maternal inheritance and various gene interactions

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Unit 9 Diversity of Life Forms

• Different protozoan and bacterial diseases.
• alpha-beta-gamma taxonomy.
• binomial nomenclature.
• The evolutionary relationships among taxa
  (graphical or phylogenetic tree, common ancestor
  based questions expected).
• Hierarchical taxa.
• common parasite and pathogens for different hosts.

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Unit 10 Ecological Principles

• Rules in Ecology like Bergmanns rule, Allens
  rule, Glogers rule, Yodas law.
• Pyramids of energy and population, thermal
  stratification in lentic ecosystem
• Population Ecology r and k selection, Hardy
  Weinberg Law, gene pool, Concepts and rate of
  change in gene frequency through natural
  selection, survivorship curves, population
  characteristics and regulation, population growth
  curves, metapopulation, terms like demes,
  dispersal, interdemic extinction, age-structured
  population are important.

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Unit 10 Ecological Principles

• The structure of an ecological organization, like
  organisms, population, community, ecosystem,
  biomes, and biosphere. It is required to have a
  good knowledge of the major biomes of the world.
• Basic Ecology Fundamentals of Ecology by Eugene
  P. Odum.
• Biosphere reserves and sanctuaries in different
  states of India, the difference between
  adaptation and acclimatization, recent concerns
  like anthropological activities, global warming,
  environmental pollution, monitoring, its effects
  on biodiversity, Project Tiger.
• Structure and components of different types of
  ecosystems like terrestrial, forest, grassland
  and aquatic.

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Unit 11 Evolution and Behavior

• Phylogenetic tree and cladogram, molecular
  evolution, molecular divergence, clocks, tools,
  protein, and nucleotide gene analysis, new
  protein or gene origin, duplication, divergence.
• The concept of speciation types such as
  allopatric, parapatric and sympatric
• Genetic drift changes through natural selection.
• Ensure you know all the types of selection like
  directional, stabilizing and disruptive
• Concept clarity should be there about the
  founders effect and bottleneck effect

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Unit 11 Evolution and Behavior

• Natural selection Lamarck concept, Darwinism,
  adaptation, variation, struggle, fitness, the
  spontaneity of mutation.
• Geological time scale Ontology, evolutionary
  history, Major events in different eras, periods,
  epochs. You can use some mnemonic code to
  memorize them.
• Concept of Oparin and Haldane Experiment of
  Miller.
• Adaptive radiation, endosymbiotic theory.

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Unit 12 Applied Biology

• Various bioresources, and uses in biodiversity,
  bioremediation and phytoremediation, purpose and
  the different organisms involved.
• You may expect some experimental questions on
  marker-assisted selection breeding of qualitative
  and quantitative traits or for disease resistance
  in plant breeding.
• Agrobacterium-mediated gene transfer process.
• Gene therapy.
• Different tissue culture requirements for both
  plants and animals.
• Biosensors.

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Unit 13 Methods in Biology

• mmunological techniques such as ELISA, RIA,
  western blot, immunoprecipitation, flow
  cytometry, immunofluorescence microscopy, FISH
  and GISH.
• T-test, X2 test
• Recombinant DNA technology RNA, DNA and proteins
  isolation, separation and analytical techniques
  (1D and 2D gel electrophoresis, isoelectric
  focusing gels), cloning,
• Recombinant protein expression using various
  bacterial, animal and plant vectors (cosmid, BAC
  and YAC vectors), genomic and cDNA libraries.

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Unit 13 Methods in Biology

• Statistical Methods Mainly questions will be
  asked from probability distributions such as
  Binomial, Poisson and normal.
• Biophysical methods such as UV/visible,
  fluorescence spectroscopy, circular dichroism,
  NMR, ESR spectroscopy, X-ray diffraction, NMR,
  different types of mass spectrometry.
• Electrophysiological methods Single neuron
  recording, patch-clamp recording, ECG, Brain
  activity recording, PET, MRI, fMRI, CAT.

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Thank You!