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Histopathology Laboratory


Histopathology Laboratory: How to Choose the Best Stain for your Slides? – PowerPoint PPT presentation

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Title: Histopathology Laboratory

How to Choose the Best Stain for your Histology
Histopathology Laboratory
  • Using stains in microscopy is a routine part of
    the daily operations in a histopathology
    laboratory. The purpose of using these stains is
    to enhance contrast and highlight the structures
    in the slides, and thus to get accurate results
    in the tests.
  • However, choosing the best stain for accurate
    results isnt always easy. This is because there
    are numerous stains in histology and selecting
    the best is cumbersome.

Here we give you a glimpse of the commonly used,
and some specialized stains, their purpose, and
utility to guide you in selecting the right stain
for your histology slides.
The Most Commonly Used Stains
Haematoxylin and eosin are the first and most
common stains used in most histopathology labs.
Both these stains are used for routine tissue
study where you dont need much detail.
  • Haematoxylin stains all the nucleic acids (like
    DNA and RNA), and eosin stains the basic
    structures like muscle or red blood cells.
  • Haematoxylin stains the cell nuclei as bluish
    purple while eosin dyes other cell structures
    pink or red.

Specific Stains for your Histology Slides
1) Gram Stain
  • Stain the slide with crystal violet and let it
    stand for a minute.
  • Gently rinse the slide with tap water or
    distilled water from a wash bottle and smear it
    with Grams iodine and potassium iodide solution.
    Let it stand for one minute.
  • Gently rinse the slide with tap water or
    distilled water from a wash bottle and
    decolourise with alcohol and acetone.
  • Follow this by counter-staining with a red or
    pink stain and let it stand for 45 seconds.

2) Congo Red
  • A stain which was once used in textile industry
    has now become popular in the histopathology
    laboratory for detecting amyloid fibres. These
    fibres get stained as a pale orange-red colour
    with the Congo stain.
  • Detection of amyloid fibres is important for the
    diagnosis of diseases like Alzheimers,
    Parkinsons, and Diabetes.
  • With routine stains like haematoxylin and eosin,
    its not possible to detect bacteria, amyloid
    fibres and other details in the study tissue.
    Thus, these specific stains must be a part of
    your histopathology laboratory.

  • If you dont have accesses to these stains or get
    stuck with any process in the lab, feel
    comfortable to outsource your work to a
    knowledgeable lab.
  • Exploit the histology services of a reputed lab
    to save money, time, and get high-quality slides
    with accurate results. Competent histology
    services have an experienced lab team to assist
    you in your daily microscopy operations and make
    your investments worthy.

Professional Histology Services
To Know More
  • Contact Us
  • Email ID project_at_cognibrain.com
  • Phone 044-49595223
  • Visit us https//www.cognibrain.com/
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