Bacterial Expression Systems (E. coli / Bacillus)

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Bacterial Expression Systems (E. coli /
Bacillus)
Creative BioMart
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Bacterial expression systems have been proved to
be the preferred option for laboratory
investigations and initial development in
commercial manufacture in comparison with various
other expression systems. The advantages of
bacterial expression include thorough
undersanding of the system, low cost, high
expression yield, ease of scale-up, and short
turnaround time.
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Creative BioMarts E.coli expression platform
collects various expression vectors available
with different N- and C- terminal tags and
different host strains to ensure the selection of
the most efficient system. As for insoluble
proteins, our experienced PhD-level scientists
have developed an effective protein refolding
system consisting of optimized refolding buffer
and prodedure. As for fermentation scale, we have
from 5 to 20,000 liters of bioreactors available
to meet your requirements.
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Advantages of bacterial expression systems

• Simple, well-understood genetics
• Easy to manipulate
• Fast expression with short doubling time
• Easy to purify from inclusion bodies
• Well-established labeling protocols for stability
  studies
• Established regulatory track record
• Easy to scale up by fermentation
• Minimal culturing cost

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In the latest decade, gram-positive Bacilli
strains have become very popular hosts for
industrial and pharmaceutical protein production.
Many pharmaceutically relevant proteins were
successfully expressed in Bacilli strains. In
contrast to the well-known E.coli, Bacilli
strains have the general advantage that its outer
membrane has no LPS. Furthermore, the Bacilli
strains are attractive hosts because of their
natural secretion capacity, which can export
proteins directly into the extracellular medium.
Creative BioMarts Bacillus subtilis system is an
ideal option for the expression of monomeric
protein products at high expression levels,
combined with our proprietary fermentation
protocols allow for fast process development and
scale-up to support early clinical testing.
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Our extensive experience and flexible strategies
enable Creative BioMart to provide high quality
proteins in scales and purity levels tailored to
your research.
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