Molecular diagnostics of viral infections

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Marion BarendsLaboratory Scientist

• Molecular diagnostics of viral infections
• Avian Influenza

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Molecular Diagnostics of viral infections

• Why molecular diagnostics
• Quick
• High sensitivity and specificity
• Ability to subtype different virus strains
• Low biohazard level in laboratory

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Overview of Virus detection
Sample (swab, nasal aspirate, ..)
Viral RNA extraction
Viral RNA
Reverse transcription
First-strand cDNA
Real-time PCR
PCR product
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Real Time PCR

• Detection of virus specific cDNA by Real-Time PCR
• higher sensitivity
• less time consuming
• Influenza A or B
• If influenza A, than subtyping for H5, Asian H5,
  H3, H9, N1
• If not Influenza A or B
• multiplex PCR to detect respiratory viruses

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Laboratory minimizing risk of contamination
Mix
amplification
RNA/DNA isolation
Clinical samples
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Media / Mix lab
Microbiology
RNA/DNA isolation
ELISA
DNA amplification analysis
Entrance
Malaria
Office