Raman Spectroscopy of AFGP in D2O Adsorbed on Mica

1
Raman Spectroscopy of AFGP in D2O Adsorbed on Mica

• Jennifer E. Sanders
• Fisk University REU/SRP Participant

Partner Paul Samuels Assisted by Vincent
Alexander, George Turner Advisors Dr. M. Guo,
Dr. Y. Cui, Dr. A. Burger
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Introduction

• What is AFGP?
• Purpose of Research

• Hydrogen bonding
• Why D2O?

3
Theoretical. The Bond Stretch Vibration Model is
used to predict the change in the vibration
frequencies from O-H to O-D. In this model, the
atoms behave as masses connected to a spring. The
effective force constant and the reduced mass of
the vibrating constituents are used to determine
the vibration frequencies. The vibration
frequencies are inversely proportional to the
atomic masses.
4
Theoretical
5
Experimental
6
AFGP
Photograph of the AFGP sample on mica substrate,
in inner area of sampleshowed new peaks.
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AFGP in D2O and AFGPs in H2O
Raman spectroscopy comparisons of AFGPs (6,7,8)
in H2O and AFGP-8 in D2O without new peaksthese
spectra taken to observe the affect of deuterium
on the peaks on the AFGP spectra very subtle
changes, small shifts occurred, as well as peak
intensity differences are seen in the spectral
regions (a), (b) and (c), collected under 11mW
laser beam and 100X objective for 30 seconds
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AFGP in D2O and AFGPs in H2O

• Changes in Raman Spectroscopy
• A) peak due to D2O
• B) peak shifted in D2O
• C) not all of the O-H was removed

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AFGP with New Peaks
Raman spectroscopy of AFGP, mica, AFGP/D2O,
AFGP/H2O showing new peaks under 11mW laser beam
and 100X objective for 30 seconds collection

• AFGPs in H2O 1002 cm-1, 1155 cm-1, 1200 cm-1,
  1356 cm-1, and 1510 cm-1
• AFGP in D2O 1002 cm-1 and 1356 cm-1

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Conclusion

• New peaks are found in the inner area of the
  AFGPs samples the outer edges do not show new
  peaks.
• 1002 cm-1 (possibly from sugar ring)
• 1356 cm-1 (possibly COO- bond)
• 1155 cm-1 (possibly C-OH bond)
• 1200 cm-1 (possibly C-O-C bond)
• 1510 cm-1 (possibly NH3 bond)

possibly connected with or related to hydrogen
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References

• 1 Ben, Robert N., et al. Direct Observation of
  Antifreeze Glycoprotein Fraction 8 on
  Hydrophobic and Hydrophilic Interfaces Using
  Atomic Force Microscopy. Langmuir 19(2003)4740
  4744
• 2 Y. Yeh and R. Feeney, Chemical Reviews 96,
  no.2 (1996)601 616
• 3 Lavalle, Ph., DeVries, A.L., Cheng, C.C.,
  Scheuring, S., Ramsden, J.J. Direct Observation
  of Post adsorption Aggregation of Antifreeze
  Glycoproteins on Silicates. Langmuir (2000)5785
  5789
• 4 Stefanic, I., Bonifacic, M., Mobacek, V.,
  Furic, K. Raman Spectroscopic Study of H2O and
  D2O Water Solutions of Glycine. Journal of
  Molecular Structure 276 (1992) 39 44
• 5 Yoshio Tomimatsu and James R. Sherer, Yin Yeh
  and R. Feeney. Raman Spectra of a Solid
  Antifreeze Glycoprotein and its Liquid and Frozen
  Aqueous Solutions. Journal of Biological
  Chemistry 251, no.8 (1976) 2290 2298
• 6 Harding, M. M., Audergurg, P.I., Haymet,
  A.D.J. Antifreeze glycoproteins from Polar
  fish. Eur J. Biochem 270 (2003) 1381 1392
• 7 Thomas, Jr., G. J., Benevides, and J.M.
  Characterization of DNA structures by Raman
  Spectroscopy high-salt and low-salt forms of
  double helical poly (dG-dC) in H2O and D2O
  solutions and application to B, Z, and A-DNA.
  Nucleic Acid Research 11, no.16 (1983) 5747
  5760
• 8 Infrared and Raman spectroscopy of
  biological materials / edited by Hans-Ulrich
  Gremlich, Bing Yan., New York Marcel Dekker,
  c2001.

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Acknowledgements

• I would like to acknowledge that this research is
  supported by the National Science Foundation and
  its Research Experiences for Undergraduates
  program through Grant number DMR-0453562, and by
  National Science Foundation Center for
  Biophotonics Science Technology at University
  of California, Davis under Cooperative Agreement
  No. PHY 0120999. Dr. Mingsheng Guo, Dr. Yunlong
  Cui, Dr. Arnold Burger, and Mr. George Turner of
  Fisk University are thanked for supplying the
  sample materials, the Raman Spectrometer, and the
  assistance throughout the program and in the
  labs. Also, Mr. Paul Samuels, my partner in the
  research is thanked for his insight and his
  perspective throughout the research experience.
  Lastly, Fisk University REU/SRP under Dr.
  Collins, Mr. Cyrus Pour, Mr. Kent Wallace, Ms.
  Tiffany Crenshaw, thank you for guiding this
  research opportunity and making it an experience.