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Coral Bleaching

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The total concentration of chlorophyll a in g/ml of sample 1 was 2.4. ... Protein Concentration: 23.13 mg protein/g wet wt. Our Experiment ... – PowerPoint PPT presentation

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Title: Coral Bleaching


1
Coral Bleaching
  • A Scientific Investigation by
  • Sherrie Engram
  • Matthew Grambow
  • Emily Jeng
  • Adam Rynalski
  • Johanna Said

2
Last Week
  • We tested our tanks temperature, salinity,
    zooxanthellae density, chlorophyll concentration,
    and protein concentration.

3
Last Weeks Data
  • Class Control Tank
  • -Salinity 34ppt
  • -Temperature 25C
  • Group One Tank (Were 1)
  • -Original Salinity 42ppt
  • -Our original salinity was above the class
    control salinity, so we removed 1.5L seawater
    and added 1.5L freshwater to equalize.
  • -Modified Salinity 34ppt
  • -Temperature 25C

4
Zooxanthellae Density
  • We took two samples of three polyps each,
    homogenized them in two milliliters of seawater,
    and calculated the zoox density based on the
    averages of the two results.
  • To calculate the zoox counts, three group members
    each performed five counts, using the
    hemocytometer. We then averaged all thirty
    counts from both sample to create the Average
    Zooxanthellae Counts value.
  • Average polyp weight .0295g
  • Average Zooxanthellae Counts 31.7
  • Average Zooxanthellae Density 5.38 x 108 zoox/g

5
Chlorophyll Concentration
  • Sample 1s Absorbance was
  • 665 .075
  • 647 .022
  • 630 .035
  • Sample 2s Absorbance was
  • 665 .033
  • 647 .015
  • 630 .038
  • We found the Ca of Sample 1 to be .852. The Ca of
    Sample 2 was .364.
  • The total concentration of chlorophyll a in µg/ml
    of sample 1 was 2.4. The total concentration of
    chlorophyll a in µg/ml of sample 2 was 1.03.
  • The average concentration of chlorophyll of the
    two samples was 3.42 µg/ml.

6
Protein Concentration Data
7
Standard Protein Curve
8
Protein Concentration Data
  • Residual line equation y1.0137x 0.1799
  • Polyp absorbance 0.568
  • Protein Concentration 23.13 mg protein/g wet wt

9
Our Experiment
  • Null Hypothesis Temperature will have no effect
    on coral bleaching.
  • Alternate Hypothesis If the temperature of the
    seawater is raised, it will induce coral
    bleaching.

10
Experimental Design
  • We plan to raise the temperature in the tank from
    25C to 31C over three weeks.
  • Every week, we will measure the zooxanthellae
    density, chlorophyll concentration, and protein
    concentration.
  • Our class control tank will be kept at 25C for
    the extent of the experiment.
  • Both tanks will be kept in equivalent salinity
    and light conditions. Both coral samples will
    have equivalent masses.

11
Our Experiment
  • At the end of last class we attempted to raise
    the temperature of our tank to 28C, using one
    heater.
  • On Thursday, we found that the temperature of the
    tank had decreased to 24C. To assist in the
    water temperature control we added another water
    heater and set both heaters to 27C.

12
T E C H N I C A L DIFFICULTIES!!!
  • After adding the second water heater to the tank,
    severe overheating occurred in our tank.
  • The temperature in the tank, estimated to be
    about 33 degrees Celsius, killed 100 of our
    coral.

13
Whats Next?
  • As a result of this setback, we plan to restart
    the experiment.
  • Today we are planning to set the temperature to
    28 degrees Celsius using only one water heater.
    We plan to closely monitor the temperature and
    the corals response to this temperature
    throughout the week.
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