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Coral Bleaching

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Increase amount of oil pollution in the sea has resulted in coral ... http://www.springerlink.com/app/home/main.asp?wasp=570ea5e7aa8d40c58a851234 ad2004b8 ... – PowerPoint PPT presentation

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Title: Coral Bleaching


1
Coral Bleaching
  • By
  • Nathalie Regalado
  • Saskia Medina
  • Cristina Lopez
  • Betty Angerville
  • Shannon Hahn

2
Effects of Oil Pollution on Corals
3
Background Information
  • Increase amount of oil pollution in the sea has
    resulted in coral bleaching in which corals
    release the algae from their tissues. When coral
    reefs are exposed to oils it can be affected in
    many different ways. Oil can cause decrease in
    growth, decrease in reproduction and colonization
    capacity, negative effects on feeding and
    behavior, and alterations of secretory activity
    of mucous cells to the coral reefs.
  • IPIECA- International Petroleum Industry
    Environmental Conservation Association.
    http//www.ipieca.org/downloads/oil_spill/oilspill
    _reports/Vol3_summary.pdf

4
Hypothesis
  • H1 Oil pollution will cause coral bleaching.
  • H2 Oil pollution will have no effect on corals.

5
  • The Problem of Mixing Oil and Water
  • http//pims.ed.ornl.gov/BlkSeawebsite/ipieca_repor
    t_dispersants.pdf
  • Natural Dispersion Waves break up the oil
    slick, forming oil droplets that become suspended
    in the water. The majority of this oil droplets
    will flout back to the surface, but a small
    portion of tiny droplets with neutral buoyancy
    will remain dispersed in the water almost
    indefinitely
  • Water-in-oil emulsification The mixing actions
    of waves can cause water droplets to be
    incorporated into the oil.

6
Protocol
  • http//www.springerlink.com/app/home/main.asp?wasp
    570ea5e7aa8d40c58a851234ad2004b8
  • Exposure Time
  • Test 1 Control
  • Test 2 3 hours
  • Test 3 48 hours
  • Test 4 Week
  • www.elsevier.com/locate/marpolbul
  • Oil concentrations in the tank suggested at 500g
    of oil (motor oil)/5000L which refers to a ration
    of 0.1g/L. Due to our 8 L tank, concentration
    will be 0.8g.

7
Experimental Control
  • The control group will be kept under the
    conditions as the experimental group, except
    exposure to oil.

8
  • Three ways of Monitoring Coral Stress
  • -Measuring zooxanthellae Density
  • -Measuring Amount of Protein
  • -Measuring Amount of Chlorophyll

9
Last Weeks Experiments
Measuring Amount of Protein
10
Procedure for Protein Measurement
  • Spectrophotometer
  • Blank 100 µL of Distilled Water and 5 mL of
    Reagent.
  • Re-zero the machine
  • Five different concentration of protein
  • Total of 10 known samples
  • Two unknowns
  • Preparing Unknowns
  • Cut about 4 polyps and weigh them on the machine
    0.0624 g
  • Add 2 mL of seawater
  • Homogenize for 20 seconds
  • Add 100 µL of homogenized solution plus 5 mL of
    reagent
  • Vortex

11
Data
12
(No Transcript)
13
Results
  • Average absorbance of two unknowns0.454
  • Calculated concentration0.665 mg/mL
  • Calculated amount of protein 21.3 mg of
    protein/g of wet weight
  • Class average12.7 mg of protein/g of wet weight
  • We are 8.6 above average

14
The End
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