Immunological algorythms in solid organ transplantation and bone marrow transplantation

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Immunological algorythms in solid organ
transplantation and bone marrow transplantation
Fundeni Centre for Immunogenetics Virology

• Ileana Constantinescu

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Fundeni Centre for Immunogenetics Virology
VIROLOGICAL ASSESSMENT Both donor and
recipient are tested for VHB, VHD, VHC, HIV 1/2,
CMV, EBV, HSV 1 and 2, VZV, HTLV 1/2 , rubella
virus, toxoplasma gondii and chlamydia.    Methods
Indirect diagnostic tests (serological) Direct
diagnostic tests, molecular biology tests (PCR,
RT-PCR).                  
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IMMUNOGENETICS  HLA
Typing by molecular biology methods PCR  
SSOP- sequence-specific oligonucleotide probe
hybridization (medium resolution ) SSP
sequence-specific primers (high resolution) SBT
(the highest available resolution) KIR
Genotyping SSP  Cytokine gene polymorphisms SSP
Cross- match - CDC AHG - ELISA -
LUMINEXAnti-HLA antibody detection and
identification - CDC - ELISA  - LUMINEX
 
Fundeni Centre for Immunogenetics Virology
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Bone Marrow Transplantation
Fundeni Centre for Immunogenetics Virology

• HLA cls I and II SSP high resolution
• HLA SBT A, B, C, DRB1, DQB1, DPB1
• Crossmatch CDC, ELISA, Luminex
• KIR genotyping
• Cytokine gene polymorphisms

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Liver transplantation
Fundeni Centre for Immunogenetics Virology

• HLA cls I and II SSO low medium resolution
• Crossmatch CDC, ELISA, Luminex
• Cytotoxic antibody- screening
• - identification

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Heart transplantation
Fundeni Centre for Immunogenetics Virology

• HLA cls I and II SSP medium resolution
• - SSO low medium
  resolution
• Crossmatch CDC, ELISA, Luminex
• Cytotoxic antibody (ELISA, Luminex)
• - screening
• - identification
  

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Cornea transplantation
Fundeni Centre for Immunogenetics Virology

• HLA cls I and II SSP medium resolution
• HLA cls I and II SSO low medium resolution
• Crossmatch ELISA, Luminex

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EQAs
Fundeni Centre for Immunogenetics Virology

• UK NEQAS
• Balkan EPT scheme
• Medical Univ. of Wienna EPT scheme
• National EPT scheme for HLA DNA

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Conf. Dr. Ileana Constantinescu
Fundeni Centre for Immunogenetics Virology

• Seful Centrului de Imunogenetica si Virusologie
• Institutul Clinic Fundeni
• Sos. Fundeni, Nr.258, sector 2, Bucuresti
• Tel/Fax [phone]
• Interior 021 318 04 00 /int. 2276
• Mobil 0744 341 984
• e-mail ileanasci_at_yahoo.com
• constantinescu.ileana_at_gmail.com
• Web-sitewww.imunogenetica.ro

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MULTUMESC !