The Hong Kong Polytechnic University

1
NEW ORIENTATIONS OF RESEARCH IN CHINESE
MEDICINES ?????????

• The Hong Kong Polytechnic University
• ??????

2
Extended Platform ???????
PolyU Institute of Modern Chinese
Medicine ??????????? PearL Materia Medica
Development (Shenzhen) Ltd. PMMD ????????(??)???
? ?? State Key Laboratory of Chinese Medicine
and Molecular Pharmacology, Shenzhen,
China ???????????????????? (???????????????)
3
Introduction ??

• PMMD is the subsidiary of The Hong Kong
  Polytechnic University (PolyU) to expand our
  capacity in the research and development of new
  drug and the related technologies
• ???????????,???????????????
• It was set up in Shenzhen Hi-tech Industrial Park
  in July 2001 and has been fully operated since
  April 2002
• ????????????,01?7???,02?4???????
• It is an upgraded research platform for drug
  discovery and providing contract research
  services to the industry
• ????????????????????,???????????????

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Situated at the southern end of Hi-tech
Industrial park in Nanshan District of Shenzhen
???????????
Invested by The Hong Kong Polytechnic University
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Mission ??

• Modernization of Traditional Chinese Medicine
  (TCM) in Hong Kong and the Chinese mainland in
  formulation, quality control, safety and efficacy
  evaluation to enhance the acceptance of TCM by
  the global market
• ??????????????????????,??????????????,???????????
  ??
• Commercialization of bio-chemical technologies
  and promotion of Hong Kong as a research and
  development (RD) centre for TCM
• ??????????,?????????????
• Promotion of RD of innovative medicine and
  advanced pharmaceutical technology in the
  industry
• ??????????,???????????

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Standard Laboratory with an total area of 2000 m2
????? 2000 ???
SPF Animal Lab ????? ?????
Molecular Pharmacology Lab ???? ???
Analytical Lab ?? ???
Pharmacology Lab ?? ???
Toxicology Lab ?? ???
Formulation Lab ?????
TCM Chemistry Lab ???? ???
Conference Room ???
Chiral Lab ?????????
NMR ?? ???
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Research Facilities ????
TCM Formulation ??
TCM Chemistry ??
Toxicology Studies??
Pharmacology Studies??
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TCM Chemistry ????
9
TCM Formulation ????
10
Toxicology and Pharmacology Studies???????
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SPF Animal Laboratory ???????????
SPF Animal Laboratory is built according to the
National Standard of the Quality of Laboratory
Animal of PRC (1999) ????????????and is
accredited the Guangdong SPF Grade Laboratory
Animal Facility Certificate ???????????????????
(2002-2005)
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(No Transcript)
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Key Personnel??
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Prof. Albert S. C. Chan ??? ??
Prof. T. H. Chan ??? ??
Prof. Georges M. Halpern
Dr. M. S. Wong ??? ??
Prof. K. Y. Wong ??? ??
Prof. S. L. Chen ??? ??
Prof. D. J. Yang ??? ??
Prof. Z. M. Qian ??? ??
Prof. T. Ye ?? ??
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Scope of Research????
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• Raw herb resources survey / Authentication of
  raw materials (distinguish between confused, fake
  substitute items)
• ????/???? (??????????)
• Quality assessment on raw materials and
  preparation of Chinese Medicine ???????????
• Establishment of quality control standards and
  biological indicators ?????????????
• Isolation and structural elucidation of the
  active components for bioactive screening ??????
• Application of modern technologies in formulation
• ?????????
• Efficacy and toxicology studies of TCM
  formulations
• ????/????????

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• Authentication / Quality Assessment
• ???? / ????/ ????

• Thin-Layer Chromatography (TLC)
• ?????????
• High-Performance Liquid Chromatography (HPLC)
• ???????
• Gas Chromatography (GC)
• ??????
• Qualitative Identification ??
• (Chromatographic Fingerprint evaluation)
• Quantification of marked ingredients ??
• (UV, TLC scanning, HPLC, GC)

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Quality Control of TCM ??????

• Good Agricultural Practice (GAP) in cultivation
  of raw herbs
• ???????????????
• Application of chemical (HPLC) and DNA
  fingerprinting to provide comprehensive component
  analysis
• ????????????????

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Herbs with Comprehensive Studies ????????

• Rhizoma Cimicifugae ??
• Fructus Schisandrae ???
• Herbal Dendrobii ??
• Radix Astragali ??
• Ramulus Uncariae cum uncis ??

• Radix Puerariae ??
• Herba Epimedii ???
• Fructus Alpiniae oxyphyllae ??
• Radix Salviae miltiorrhizae ??
• Rhizoma Polygonati odorati ??
• Radix Rubiae ??
• Semen Cassiae ???

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• Screening for Active Ingredients
• ??????

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Hong Kong Chinese Materia Medica Standards ???????

• Content of study ????
• General description ????
• Microscopic identification ????
• Chromatography (HPLC, TLC, GC) ????
• Determination of heavy metal, pesticide residues,
  mycotoxins, foreign matter, ash, water content
  and extractives ???????????????????????????
• GC and HPLC fingerprints ???????????

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• Spica prunellae Common selfheal???

23

• Rhizoma atractylodis macrocephalae??

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Application of Modern Formulation Technologies
?????????

• Osmotic pump controlled release technology ??????
• Macroporous resin refining technology ????????
• Phospholipids complex technology ???????
• Solid dispersion technology ??????
• Cyclodextrins inclusion technology
• Supercritical fluid extraction ???????
• Spray-drying, freeze-drying, film-coating
• ??????,??????, ??????

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Efficacy test arthritis product ????-?????

• Testing Models?????? (???) ??????
• Adjuvant-Induced Arthritis in Rats (30 days)
• ????????????
• measure the primary and secondary paw edemas
  and the arthritis index ?????????,???????????,????
  ??
• 2. Cotton-Induced Outgrowth in Rats (14 days)
• ???????????
• measure the dry weight of cotton pellet induced
  granuloma
• ??????,??????????
• 3. Acetic Acid Induced Body-Twist Model in Mice
  (10 days)
• ????????????
• observe the frequency of body twisting in mice
  after the injection of acetic acid mixture
  ??????????,????????????

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Efficacy test slim product ????-?????

• Animal Model (Nutritious Obesity)
• ????????????????
• SPF grade Rats feeding with high energy diet for
  30 days Observe for
• the Testing Criteria after the administration of
  product for conse. 28 days
• ???????SPF???30??,??28??????
• Testing criteria (????)
• (1)Body Weight (Daily) ?? (??)
• (2)Lees Index Lees ??
• (3)Weight of Adipose Tissues ????????
• (4)Determine the size of fat cells ?????????
• (5)Blood Lipid (serum CHL, TG, HDL, LDL)
• ?? (???????????????????
• ??????????????)

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Safety Evaluation ?????

• Acute Toxicity
• ??????
• Chronic Toxicity
• ??????
• Genetic Toxicity
• ??????

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Product Development????
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• Cardiovascular diseases?????
• JCM01021/JCM01023
• Single compound??
• For myocardial ischemia/angina ??????/???
• Poor bioavailability and only available in
  injection form??????,?????
• Modification by a) phospholipids complex
  formation b) structural modification ????
  a)????b)????
• US, PCT and China patent filed
  ??????PCT?????

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• JCM01022
• Compound formula consisting of the active
  fractions of two herbs ??????????????
• For hyperlipidemia??????
• Good comprehensive blood lipids modulating
  effect??????????
• China and PCT patents filed
  ??????PCT??

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• Rheumatoid arthritis??????
• Compound formula based on triptolide
  ???????????
• Triptolide is an effective immunosuppressant with
  strong adverse drug effect??????????????,????????
• To screen for suitable active compounds in herbs
  to reduce the toxic effects of triptolide and
  increase the inflammation inhibitory
  effect???????????,???????

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Development of New Drug/ Functional Food ????/
?????

• RD focus on Chronic Diseases / Autoimmune
  Diseases with outstanding beneficial effects when
  using Chinese Medicines
• Cardiovascular Diseases
• Myocardial ischemia
• Hyperlipidemia
• Rheumatoid Arthritis / Arthritis
• Osteoporosis
• Cancer
• Gout
• Primary Screening for the New Drug Development

• ??????????????????? / ??????
• ?????
• ??????
• ????
• ??????? / ???
• ?????
• ??
• ??
• ?????????

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Development of CVD Drug ???????
GAP Study ???????????
Medicinal Plant Survey??????
Safety Evaluation ????????
Drug Metabolism ?????
Pharmacology Study ?????
Clinical Testing ??????
Registration??
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Future Direction??????
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• RD of functional food based on Chinese Medicine
  ??????/??????
• Application of advanced technology in TCM
  research
• ???????
• 3) New interdisciplinary applications of Chinese
  Medicine
• ????????

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Interdisciplinary Study ??????

• Structural modification of naturally occurring
  compounds to increase the efficacy and/or
  decrease the toxicity
• ?????????????/???? (Organic synthesis)
• Textiles (including mosquito nets) impregnated
  with insect repellent Permethrin, using
  nanotechnology ??????? (Textile)
• Control of cataract development in aged subjects
  with TCM-derived extracts ????????????
  (Optometry)
• Cordyceps extracts to improve endurance and
  performance in athletes
• ???????????????? (Sports Nutrition)
• Development of a TCM-derived fungicide to control
  mold invasion and dissemination from filters of
  sealed building air-conditioning systems
  ?????????????????? (Building)

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Mind Map for the Development of Chinese Medicine

• Existing Strength

Research Directions
Deliverables
Advanced technology for TCM research

• Area of Excellence
• Drug discovery and Synthesis
• Chinese Medicine Research

Upgrade the standard of technology used for
testing manufacturing of TCM products
New interdisciplinary applications of Chinese
Medicine
Novel and innovative applications of TCM
State Key Laboratory in Chinese Medicine and
Molecular Pharmacology
Development of high-quality TCM-based functional
food targeted for both national and
international market
RD of functional food based on Chinese Medicine
Development of TCM based drugs targeted for both
national and international market
Interdisciplinary Research Expertise in
biology, chemistry, biotechnology, engineering,
health sciences and food sciences at PolyU
New drug development based on Chinese Medicine
Interdisciplinary training platform for students
Provide well-trained professionals for
supporting development of the TCM industry
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Achievement??
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State Key Laboratory ???????

• PMMD has been recognized as the
• Shenzhen Key Laboratory for Formulation,
  Toxicology
• and Pharmacology since October 2003
• ???2003?10?????
• ???????? - ????? ? ??

It has been further recognized as the State
Key Laboratory of Chinese Medicine and Molecular
Pharmacology, Shenzhen, China since January
2005 ???2005?1????? ????????????????????
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Development Roadmap ?????
AOE in Drug Discovery and Synthesis AOE in
Chinese Medicine and Further Development
Institute of Modern Chinese Medicine Institute of
Materia Medica
Shenzhen Key Laboratory in TCM Formulation

• 2002 2003 2004
• 2005 2006 2007 2008 2009 2010 2011

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Contact (Hong Kong) ?????
??? ??? ?? ?? 852-34008694 ??
trhelen_at_inet.polyu.edu.hk ??
www.pearlsz.com ?? ???????????? Y829?
Contact Person Ms. Helen Tam Telephone no
852-3400-8694 Email trhelen_at_inet.polyu.edu.
hk Website www.pearlsz.com Address Y
829, The Hong Kong Polytechnic University,
HungHom, Kowloon, HK
42
Contact (Shenzhen) ?????
??? ??? ?? ?? 86-755-26737462 ?? bcjoyce_at_i
net.polyu.edu.hk ?? www.pearlsz.com
?? ???????? ???????R2-A?6/F
Contact Person Ms. Joyce Ho Telephone no
86-755-26737462 Email bcjoyce_at_inet.polyu.ed
u.hk Website www.pearlsz.com Address 6/F
A District R2 Building, Hi-tech Industrial
Park, Nanshan District, Shenzhen, Guangdong, China