Prsentation PowerPoint - PowerPoint PPT Presentation

Loading...

PPT – Prsentation PowerPoint PowerPoint presentation | free to download - id: 2a1179-MmVlM



Loading


The Adobe Flash plugin is needed to view this content

Get the plugin now

View by Category
About This Presentation
Title:

Prsentation PowerPoint

Description:

Prsentation PowerPoint – PowerPoint PPT presentation

Number of Views:55
Avg rating:3.0/5.0
Slides: 59
Provided by: vanden57
Learn more at: http://pbil.univ-lyon1.fr
Category:

less

Write a Comment
User Comments (0)
Transcript and Presenter's Notes

Title: Prsentation PowerPoint


1
Structure, dynamique et robustesse des réseaux de
régulation transcriptionnelle
Bernard Vandenbunder
Réunion satellite JOBIM 9 Juillet 2005 Des
mesures aux modèles et des modèles aux mesures
2
un modèle simple
3
Systematic determination of genetic network
architecture Tavazoie S and Church GM (1999),
Nature Genetics, 22281-5.
4
Identifying regulatory networks by combinatorial
analysis of promoter elements Pilpel, Y.,
Sudarsanam, P., and Church, G. M. (2001) Nat
Genet 29, 153-9. .
5
(No Transcript)
6
Transcriptional regulatory networks in
Saccharomyces cerevisiae Lee and RA Young
(2002), Science 298, 799-804.
7
Les réseaux de régulation transcriptionnelle dans
un environnement non idéalisé
Peptidoglycan
Proteins
Ribosome
mRNA
DNA
From David D Goodsell, http//www.scripps.edu/pub/
goodsell/illustration/public/
8
The structure of histone-depleted metaphase
chromosomes Paulson, J.R. and Laemmli,
U.K. Cell, 12, 817-28, 1977
9
(No Transcript)
10
From Alberts et al. Molecular Biology of the
Cell Third Edition. Chapter 8, fig. 8.24
11
110 Å
50 Å
http//www.mol.biol.ethz.ch/groups/richmond/projec
ts/nucleosome
12
Multisubunit RNA polymerases.
Cramer P (2002) Curr Opin Struct Biol. 1289-97
110 Å
RNA polymerase II takes 30 seconds to one minute
to transcribe a 1 kilobase long gene
13
Quelques ordres de grandeur
102 to 103 bp 400 genes 103 to 104 bp
6000 genes, 1 à 10 minutes 104 to 105 bp
12.200 genes 10 à 100 min 105 to 106 bp
3200 genes 106 to 2.4 106 bp 62 genes
16 à 24 h
Longueur des gènes H. Sapiens
Nombre de transcrits par gène 0,1 à 106.
jusque 106 mRNA globine dans les cellules
érythroïdes
15.000 mRNA actine / noyau dans les fibres
musculaires
10 à 100 copies pour les mRNA codant pour les
facteurs de transcription
Dans une cellule HeLa, il y a environ 100 mol.
RNA pol / gène codant rRNA
14
linitiation de la transcription
Transcription factor activator
15
(No Transcript)
16
Prolifération Transformation
Myc
NTD
HDACs
CTD
Daprès Sakamuro Prendergast, Oncogene (1999),
18, 2949-54
17
(No Transcript)
18
ChIp
Fixation
Lysis, sonication and immunoprecipitation
DNA extraction
Amplification
From Shang et al., 2000 Cofactor dynamics and
sufficiency in estrogen receptor regulated
transcription Cell, 103, 843-852
19
?
, ou
et
ReChIp
1st Immunoprecipitation
2d Immunoprecipitation
DNA extraction
Amplification
From Métivier et al., 2000 Estrogen receptor a
directs ordered, cyclical and combinatorial
recruitment of cofactors on a natural target
promoter. Cell, 115, 751-63
20
(No Transcript)
21
(No Transcript)
22
Alternative recruitments, alternative cycles
Existence of a transcriptional clock
23
après linitiation de la transcription
24
régulation de la stabilité des ARNs messagers
Global analysis of stress-regulated mRNA turnover
by using cDNA arrays,
Fan, J. et al. (2002). Proc Natl
Acad Sci U S A 99, 10611-6.
Precision and functional specificity in mRNA
decay,
Wang, Y et al.
(2002). Proc Natl Acad Sci U S A 99, 5860-5 .
25
Control of gene expression during T cell
activation alternate regulation of mRNA
transcription and stability.Cheadle et al.,
(2005), BMC Genomics, 675
26
régulation par les petits ARNs messagers
27
régulation par les petits ARNs messagers
From Gottesman, S. (2002). Stealth regulation
biological circuits with small RNA switches,
Genes Dev 16, 2829-42.
28
Structure, dynamique et robustesse des réseaux de
régulation transcriptionnelle
Réunion satellite JOBIM 9 Juillet 2005 Des
mesures aux modèles et des modèles aux mesures
29
Modeling the molecular regulatory mechanism of
circadian rhythms in Drosophila Leloup
Goldbeter, BioEssays. 2000, 22 84-93
30
(No Transcript)
31
"The Circadian Clock That Controls Gene
Expression in Arabidopsis Is Tissue Specific.
Thain, S. C., G. Murtas, et al. (2002). Plant
Physiol. 130(1) 102-110.
32
A serum shock induces circadian gene expression
in mammalian tissue culture cells. Balsalobre A,
Damiola F, Schibler U. Cell. 1998 93929-37.
33
The network of time understanding the molecular
circadian system. Roenneberg T and Merrow M
Current Biol., 13, R196-204
34
The IkappaB-NF-kappaB signaling module temporal
control and selective gene activation, Hoffmann
and D. Baltimore. (2002). Science 298, 1241-5.
35
(No Transcript)
36
Oscillations in NF-kB signaling control the
dynamics of gene expression, Nelson DE and MRH
White. (2004). Science 306, 704-8.
37
Structure, dynamique et robustesse des réseaux de
régulation transcriptionnelle
Réunion satellite JOBIM 9 Juillet 2005 Des
mesures aux modèles et des modèles aux mesures
38
SF
0
0.5
2
4
8
24
h
ETS1
uPA
Collagenase
PAI-1
GAPDH
39
(No Transcript)
40
(No Transcript)
41
Gene expression regulation a noisy business
from http//www.medicine.man.ac.uk/esrg/jdavis.htm
42
Dynamic patterns of growth hormone gene
transcription in individual living pituitary
cells, Norris, A. J., et al. (2003), Mol
Endocrinol 17, 193-202.
43
Transcription occurs in pulses in muscle
fibers Newlands et al., Genes Dev. 1998,
122748-58
44
Expression de la bgal sous le contrôle du
promoteur du gène codant Tn1
45
adulte flexor digitorum brevis
embryon E 16.5 quadriceps
embryon E 16.5 tibialis anterior
46
from Becskei Serrano., Nature. 2000, 405
590-3 Engineering stability in gene networks by
autoregulation
47
Fractal dynamics in physiology Alterations with
disease and aging Ary L. Goldberger et al.
PNAS. 2002 99 24662472
48
Studies on structure, dynamics and robustness of
regulatory networks, in the context of
multifactorial diseases
49
(No Transcript)
50
A strategy of development
  • The focus on formation
  • The integration of new teams
  • The conception of a new building

51
Ecole thématique Interdisciplinaire
 Microscopie Fonctionnelle en Biologie 
Ile dOléron, 12/17 septembre 2004
Interdisciplinary workshop
Noise and robustness in transcriptional
regulatory networks
September 3/7 2005
http//www.ibl.fr/noisitransnet/
52
Modélisation et simulation des nanosystèmes
biologiques Ralf Blossey (IRI_at_IEMN),
Systems Epigenomics, Arndt Benecke (IRI_at_IHESIBL)
Structuration des surfaces de silicium
fonctionnalisées, applications à la biologie
Rabah Boukherroub (IRI_at_IEMN)
Approche protéomique et fonctionnelle du
remodelage de la chromatine Pierre Olivier
Angrand (IRI_at_IBL)
Equipe Physique expérimentale, Didier
Stievenard (IEMN)
53
(No Transcript)
54
(No Transcript)
55
(No Transcript)
56
completion July 2007
57
http//iri.ibl.fr
58
(No Transcript)
About PowerShow.com