Lentikats Biotechnology in distilleries

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Lentikats Biotechnologyin distilleries
Biotechnologies are changing the world..
Biotechnologie, které mení svet...
Nitrogen pollution specialists
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Content

• PRINCIPLES
• Principles of Lentikats Biotechnology
• Benefits of Lentikats Biotechnology
• Lentikats Biotechnology in distilleries
• 2. CASE STUDIES
• Case study molasses distillery
• Case study distillery processing sugar
  solutions
• Case study corn distillery
• 3. WHAT CAN WE OFFER?
• References and experiences, our partners

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Principles of Lentikats Biotechnology
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What is a Lentikats Biocatalyst?

• Unique shape of the matrix and the original
  principle of immobilisation enable the
  encapsulation of free enzymes or microorganisms
  (bacteria, yeasts, fungi) in a stable porous PVA
  (polyvinylalcohol) carrier. The carriers
  advantages are
• Excellent physio-mechanical characteristics that
  provide for long-term mechanical stability,
• Low biodegradability,
• Zero toxicity.
• Lentikats Biocatalyst enables easy separation of
  enzymes and microorganisms from the surrounding
  media and therefore it can be reused multiple
  times.

Biotechnologies are changing the world
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Lentikats Biocatalyst

• Advantages of using PVA as a matrix for
  encapsulation
• inexpensive, non-toxic
• polymerization under optimal conditions, cell
  conservation
• enables creation of hydrogels with excellent
  mechanical properties
• no side effects in processes
• large capacity for cell building
• easily supervised processes
• stable processes
• easy separation from bulk media

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Lentikats Lentikats

Biocatalyst
Biotechnology (our product)
(application process)
Lentikats Biocatalyst Production
Lentikats Biocatalyst Application
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How does Lentikats Biocatalyst function?

• Due to its maximum surface and at the same time
  minimum volume of the Lentikats Biocatalyst, the
  diffusion limitations associated with the
  transport of the substrate into the lens and the
  product out of the lens are significantly reduced.

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Production of Lentikats Biocatalyst
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Lentikats Biocatalyst
Production plant in Strá pod Ralskem
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Benefits of Lentikats Biotechnology
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What are the advantages of Lentikats
Biotechnology over traditional technologies?
1)
Higher biomass concentration
Reduced volume of reactors
Reaction rate
Investment costs saving
Biomass concentration
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100
100
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Operational costs saving (electricity, heat,
etc.)
Higher yields
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What are the advantages of Lentikats
Biotechnology over traditional technologies
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2)
Easy separation from bulk media
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Operational costs saving
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Repeatable use of Biocatalyst
Lower production of waste and by-products
3)
Robustness towards environmental shocks
profound change of pH level higher concentration
of toxic compounds
Applicability under extreme or stress conditions
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Lentikats Biotechnology in distilleries
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Technological Process set-up

• Legend
• Sugars media inlet
• Fermented media
• Inlet for Lentikats Biocatalyst filling
• Outlet for Lentikats Biocatalyst emptying
• Sieve separator (angle slop 15-20, aperture
  0.8-1 mm)
• Liquid fermentation broth with Lentikats
  Biocatalyst
• Liquid fermentation broth without Lentikats
  Biocatalyst
• Gear with speed change box
• Stirrer
• pH probe well
• Temperature probe well
• Antifoam agent inlet
• Liquid level measurement (stainless steel
  pressure probe)

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Which substrates can be used for Lentikats
Biotechnology?
Lentikats Biocatalysts provide a solution for
the production of bioethanol from the following
substrates

• Molasses, beet juice and sugar cane,
• Starch-containing (cereal) materials (corn,
  wheat, etc.),
• Ligno-cellulose containing materials (straw,
  pollard).

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Our products

• Lentikats Biocatalyst for the fermentation of
  sucrose, glucose and lactose solutions
  (immobilized bacteria Zymomonas mobilis, yeast
  Saccharomyces cerevisiae, Kluyveromyces sp.),
• Lentikats Biocatalyst for the 2nd step of
  hydrolyses of liquefied starch solution
  (immobilized enzyme glukoamylase),
• Lentikats Biocatalyst for the production of
  inverted sugar from molasses (immobilized enzyme
  invertase).

Energy is all around us the key is to
obtain it effectively and protect the
environment at the same time
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Case study molasses distillery
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molasses distillery (125,000 l a.a. per day,
continuous mode)
45 tons of Lentikats Biocatalyst
(TFS 48 )
41 667  m3/year a.a.
404 tons/day
130 g/L TFS
Total Fermentable Sugars
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Fermentation conditions

• Saccharomyces cerevisiae
• continuous mode
• 180 g sucrose . hour-1 . kg-1
• ethanol yield 95
• 8.4 vol. ethanol

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Economic comparison of traditional technology and
Lentikats Biotechnology for molasses distillery
Biotechnologies are changing the world..
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Reactor volume reduction reduced investments
Biotechnologies are changing the world..
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Lentikats Biocatalyst consumption (125,000 L a.a.
per day)
Biotechnologies are changing the world..
Guaranteed lifetime Assumed lifetime
Biotechnologies that are
environmentally friendly
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Profit of ethanol production at the capacity of
125,000 L a.a. per day
Biotechnologies are changing the world..
Solution for higher efficiency and stability
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Case study distillery processing sugar
solutions (sugar cane or sugar beet juice)
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Sugar distillery (125,000 L a.a. per day,
continuous mode)
45 tons of Lentikats Biocatalyst
sugar solution
41 667  m3/year a.a.
140 g/L TFS
Total Fermentable Sugars
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Fermentation conditions

• Zymomonas mobilis
• continuous mode
• 180g saccharose. hour-1 . kg-1
• temperature 30 35C,pH 4.8 5
• ethanol yield 95
• 9.02 vol. ethanol

Biotechnologies are changing the world..
Biotechnologies are changing the world..
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Economic comparison of traditional technology and
Lentikats Biotechnology for distillery processing
sugar solutions
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Lentikats Biocatalyst consumption (125,000 L a.a.
per day)
Biotechnologies are changing the world..
Biotechnologies are changing the world..
Guaranteed lifetime, assumed lifetime
Biotechnologies that are environmentally
friendly
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Profit of ethanol production at the capacity
of125,000 L a.a. per day
Solution for higher efficiency and stability
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Case study corn distillery
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Corn distillery (15,000 L a.a. per day,
continuous mode)
6 tons of LB
1.8 tons of LB
(60 of starch)
5,000,000 m3/year a.a.
39 tons/day
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Hydrolysis conditions

• glucoamylase
• continuous mode
• 600 g glucose . hod-1 . kg-1
• temperature 38 40C, pH 4.5 5
• conversion rate 95
• outlet concentration 150 g/L

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Fermentation conditions

• Zymomonas mobilis
• continuous mode
• 180g glucose. hour-1 . kg-1
• temperature 30 35C,pH 4.8 5
• ethanol yield 95
• 9.21 vol. ethanol

Biotechnologies are changing the world..
Biotechnologies are changing the world..
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Economic comparison of traditional technology and
Lentikats Biotechnology for corn distillery
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Reactor volume reduction reduced investments
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Lentikats Biocatalyst consumption (125,000 L a.a.
per day)
guaranteed lifetime, assumed lifetime
Biotechnologies that are environmentally
friendly
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Profit of ethanol production at the capacity of
15,000 L a.a. per day
Solution for higher efficiency and stability
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Other Lentikats Biotechnology Applications

• Lentikats Biocatalysts and applied Lentikats
  Biotechnology also deliver additional value and
  benefits in the following sectors
• waste water treatment nitrogen removal,
• pharmaceutics production of robust
  biocatalysts,
• food industry whey processing, production of
  special sugars carbohydrates and
  syrups.

Biotechnologies are changing the world
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What can we offer?
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Lentikats Biotechnology services

• Monitoring of specified technological procedures
  and conditions.
• Periodical monitoring of Lentikats Biocatalysts
  activity.
• Supply and replacement of Lentikats Biocatalyst
  in such periods and volumes to sustain the
  required Lentikats Biotechnology parameters.
• Disposal of the used Biocatalyst.

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What can we offer to you?

• Have we raised your interest in testing Lentikats
• Biotechnology in your own project?
• We are prepared to supply a sufficient amount of
  our Biocatalyst and provide full assistance in
  order to convince you of the obvious advantages
  our technology has when applied in your
  distillery.
• For all companies introducing new technologies,
  the first applications and end results are the
  most important ones. That is why we offer
  excellent conditions to potential clients in an
  effort to eliminate any of their risks.

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Guarantee of your investments and the investment
return

• You can test our biocatalyst easily by yourself
  with your substrate in your own plant or plant of
  your choice to see the results.
• We offer to you our assistance and full support
  at
• - pilot model installation
• - installation into an existing reactor
• Load of Biocatalyst for testing is free of
  charge.

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References and experiences, our partners
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Our know-how, references and experiences

• Production of bioethanol from sugar beet molasses
  in Bioferm Kolín distillery with a capacity of 6
  litres of absolute alcohol per hour.
• Processing of technical-technological
  documentation for the supply to an agricultural
  refinery with a production capacity of cca 3 000
  m3
• of bioethanol per year, based on our concept of
  Bio Plant 2015.
• Processing of technical documentation for the
  delivery of a distillery with a production
  capacity of cca 450 hl of bioethanol per day
  utilising corn as a raw material.
• Production of bioethanol from starch milk of type
  B in Krnov distillery with a capacity of 12
  litres of absolute alcohol per hour.
• Process optimisation and processing of technical
  documentation for a reconstruction of the
  existing distillery in India which uses cane
  molasses as a substrate.

Biotechnologies are changing the world..
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Our partners in the field of industrial
applications
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Our partners in the field of research and
development
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Thank you for attention

• Company Headquarters
• LentiKats a.s.
• Evropska 423/178
• 160 00 Prague 6
• Czech republic
• Tel. [phone]
• Fax [phone]
• E-mail info_at_lentikats.eu
• Production plant and laboratories
• LentiKats a.s.
• Pod Vinicí 83
• 471 27 Strá pod Ralskem
• Czech republic
• Tel. [phone]