Cystic Fibrosis

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Cystic Fibrosis Alzheimers PART 2
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Todays schedule
1) Cystic Fibrosis (75 min.) 2) Alzheimers
disease (45 min.)
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Grand challenge Every disease requires a
tremendous amount of interdisciplinary effort to
understand and fight it. Can this be streamlined?
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Positional cloning

• A chromosome has a single DNA molecule
• running the length of the chromosome.
• Map the gene for the trait in question in
• that DNA molecule
• identify close flanking markers
• Walk between the flanking markers
• Identify the gene in question between
• the markers

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Reconstitution Assay
homogenize centrifuge
mRNA aas
protease treatment
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incubate
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Osmosis The movement of water through
a semipermeable membrane driven by a difference
in concentration of impermeant solute on either
side.
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Cystic fibrosis By limiting Cl- efflux from the
cell, the cell is unable to hydrate mucous
membranes sufficiently.
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Paradox DF508 makes a functional Cl- channel
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BREAK TIME
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Alzheimers Disease
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Next class Cell division Cell death Cancer