Replikasyon

1
DNA Replication
2

• DNA template with synthesis of a new strand in
  the 5' to 3' direction.
• The 5' triphosphate can only be added to a free
  3'OH of deoxyribose.
• The two antiparallel strands are replicated
  simultaneously in both directions.
• RNA primers are used to initiate a new strand
• The parent strand at the 3' end of the template
  determines the daughter or leading strand in
  continuous replication.
• The parent strand at the 5' end of the template
  produces the lagging strand as short pieces of
  DNA (100-200 nucleotides in eukaryotes and longer
  in prokaryotes).
• The lagging strand fragments are called Okazaki
  fragments after their discoverer, Reiji Okazaki.
• The RNA primers are removed and the fragments are
  joined by DNA ligase.

3
Direction of replication  
4
The replication fork 
5

• Conclusions
• DNA structure predicts that base sequence
  determines replication of DNA
• If the sequence contains information, then the
  bases A,T,G, and C must somehow determine
  aminoacid sequences in proteins.
• Mutations in DNA are caused by changes in base
  sequences.