Shimizu Lab Specific Training

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Shimizu Lab Specific Training

• May 7th, 2009

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University of Minnesota Hazardous Chemical Waste
Management Guidebook

• Theres a direct link to it from the Shimizu Lab
  Website or go to www.dehs.umn.edu
• All personnel are responsible for ALL hazardous
  chemicals they work with.
• The guidebook covers how to handle, label, and
  dispose of hazardous waste.

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Chemical Spill

• Evacuate the area and shut off electrical
  equipments. Alert others using signs or tape.
• Provide Aid immediately remove contaminated
  clothing, flush skin or eyes with running water
  for at least 15 minutes, and seek medical
  attention if necessary.
• Confine the area.
• Call DEHS at 6-6002 for chemical spills of highly
  reactive or toxic, during work hours. Call 911
  during after hours.
• Clean up, using appropriate cleaning agents
  Bleach, alcohol, or the spill kit.

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Chemicals/Labels

• All chemicals should be stored in leak proof
  bottles and have leak proof secondary
  containments.
• Labels should contain the full chemical name, no
  abbreviations/chemical formula.
• List all components and percentage within the
  bottle.
• Date
• pH
• Use yellow Hazardous Waste Labels provided by
  DEHS.

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Working with Infectious Agents

• Use Autoclave trash bags for all infectious
  wastes. Autoclave at 121 degrees celsius for 60
  min.
• Red Bags can be used, however it costs 10x more
  for the University to dispose of Red Bags, and
  theyre only intended for use when there are no
  autoclaves around.
• No Sharps or Liquids in trash
• DO NOT overflow trash, fill it up to ¾ full.
• All sharps should go in Sharps containers.
• All Liquids can be decontaminated with 1 part
  bleach to 10 part liquids for 15 minutes, then it
  can go down the drain.

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Virus Hood

• Serological Pipets goes into the autoclave trash.
  
• Pipet tips goes into the bleach waste bucket.
  Everyone is responsible for emptying the tip
  waste once its full. Tips can go into the trash
  and the liquid wastes can go down the drain.
• Monitor the liquid waste container, empty it if
  more than ¾ full.
• Clean up any spills, wipe down the work area, and
  keep the hoods clutter free.
• Pipet Aids need to remain at their designated
  hoods.
• At the end of the day, leave UV light on for
  15-30 minutes, then shut off all exhaust to help
  prolong the life of the filter.

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Personal Protective Equipments

• Wear appropriate gloves for all procedures.
• Gloves can only be worn in workspaces. Not at the
  computer or outside of the lab area.
• Wear goggles and face shields when necessary.
• Lab coats or disposable gowns when working with
  hazardous chemicals and/or mice.

8
Lab Safety

• Doors cannot be blocked or propped open under any
  circumstances. Ex) LN tanks cannot block fire
  doors.
• Eye washes need to be checked once a week and
  documented.
• Safety showers are located along the main
  corridor, checked by Building Management
  annually.
• No open toe shoes.
• No shorts or loose clothings.

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SOPs

• SOP template can be obtained at
  www.dehs.umn.edu/ressafety_rsp.htm
• All SOPs should include safety measures that need
  to occur during each procedures and procedures to
  be followed in the case of any spills, exposures,
  or accidents.
• All procedures in the lab must have a
  corresponding SOP.

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Anesthetic Gas

• www.dehs.umn.edu/PDFs/anestheticgases.pdf

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Lab Cleanliness

• Keep benchtops clutter free
• Put things back in place after use.
• Wipe down counters before and after use.
• Drying racks should be cleared once equipments
  are dried.
• Everyone should help monitor the CO2 tanks for
  our incubators and notify me or change out the
  tanks when its low.

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Youre now good for another year.

• Please Sign and Date the Sign-In sheet