Towards Sensitized Lanthanide Emission via a Betalamic Acid Chromophore Isolated from Beta vulgaris.

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Beta vulgaris
Towards Sensitized Lanthanide Emission via a
Betalamic Acid Chromophore Isolated from Beta
vulgaris. Tiffany A. Brewer, Paul I. Higgs, and
Mark D. Keränen Department of Chemistry, The
University of Tennessee at Martin,
Betalamic acid
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Sensitized Emission of Ln3
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Lanthanide Cyclen Complexes (Lumophore)
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Literature Sensitizers
Mishra, Pfeuffer, Mishra, Engelmann, Mishra,
Ugurbril,and Logothetis. Biconjugate Chem 2006
17 773-780
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Potential Betalamic acid Sensitizer
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Extraction of Betacyanins
1. Red beet (homogenized or juiced) macerated
with acidified water (TFA pH 5-6) 2. Filtered,
Separated by Ion Exchange Chromatography with
Amberlite XAD-16. Elution with water (TFA pH 5-6)
followed by methanol gave fractions containing
the vivid red-violet betacyanins
Crude
Betanin
Fractionated betaxanthins and betacyanins
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Improved In Situ Generation of Betalamic Acid
?Concentrated betanin solution, excess
NH4OH(aq) 10-15 mins by UV hydrolysis completed
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Forming the Antenna
UV
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Forming the Antenna
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Acknowledgements
Dr Paul Higgs Dr Mark Keränen Carolyn
Nguyen Clint Ary Adam Thompson UTM College of
Engineering and Natural Sciences (CENS)
undergraduate research grant UTM College of
Engineering and Natural Sciences (CENS)
Instrument grant purchase of UV-Vis
(Thermoscientific evolution 300)