Lonnie Ingram, Director

1
Replacing Imported Petroleum with Renewable Fuels
such as Ethanol Made from Modern Biomass
Energy Petroleum Independence A moonshot
for this generation.

• Lonnie Ingram, Director
• Florida Center for Renewable Chemicals and Fuel
• The University of Florida

2
Florida uses 8.6 billion gallons of automotive
fuel per year.
Fossil Biomass
U.S. -- 160 billion gal fuel/yr total
imported petroleum
U. S. DOE
3
Brazil currently burns E85 in many vehicles.
There are no technology barriers to expanding the
level of ethanol in US automotive fuel.
US
5?15 Billion Gallons per yr
BioDiesel (B20) 85 Ethanol Gasoline 10 Ethanol
Gasoline
But not in Florida
4
Inedible Parts of PlantsPlant Cell Wall
Carpita and Gibeaut, 1993
5
National Research Initiative Biobased Products
and Bioenergy Research
Office of Science, Energy Biosciences Energy
Efficiency and Renewable Energy Biomass
Program
Cellulosic Ethanol
Joint USDA-DOE Biomass Initiative
Cellulosic fuels could replace 1 / 3 to 1 / 2
of US automotive fuel.
6
Florida is Number 1 in Biomass Production.

• Florida could produce over 8 billion gal of
  ethanol
• per year from biomass residues and energy crops.
• Residues Agricultural, Forestry, and
• Municipal Green Waste
• Energy crops Short rotation Trees, grasses,
  canes

Florida can be Number 1 in the Nation in
Renewable Fuel Production.
7
70 Carbohydrate woody biomass corn
Primarily pentoses, 5-carbon sugars.
Xylose and other sugars
Natures plastic glue
Black thermoplastic that can be burned to provide
energy for ethanol production.
Glucose polymer
Homopolymer of glucose, hexose, a 6-carbon sugar.
Composition of Lignocellulosic Biomass
8
HEXOSES PENTOSES
Microbial Platform
Embden-Meyerhof-Parnas Entner-Doudoroff
Pentose Phosphate
PYRUVATE
Succinate
(Zymomonas mobilis)
PEP
X
Pyruvate Decarboxylase
0.4mM (pdc)
Acetaldehyde CO2 Alcohol
Dehydrogenase
(adhB) Ethanol (95 Yield)
X
Lactate Dehydrogenase 7.2 mM (ldhA)
Pyruvate Formate-Lyase 2 mM (pfl)
X
Lactate
Acetyl-CoA Formate
CO2
H2
Acetate
Ethanol
Derivatives of E. coli and K. oxytoca
9
CellulosicEthanol

• Production of Ethanol in Florida
• 124 million tons biomass x 100 gal max
  ethanol/ton 8.0 to 12 billion gallons of
  ethanol
• Florida uses 8.6 billion gallons of gasoline/yr

10
UF Licensee Verenium Corp.Hydrolysis Testing
Facilities
1 lb. batch steam gun 2 ton per day continuous
feed
11
Osaka, Japan - Wood Waste to Ethanol
Began operation in January 1, 2007 1,000,000
liter/yr
Verenium
12
Florida can be the No. 1 State in Renewable Fuel
Production through Building World-class Academic
Programs and Research Capacity.
We have already started this process
1) Florida Center for Renewable Chemicals
and Fuels (2001) at UF 2) Center
of Excellence Biofuel Pilot Plant on campus
(2.3 million) 3) Over 15 million in
Federal grants and industry sponsored research
4) Patented Licensed UF technology to
create commercial partners 5) State
Grant to UF - 20 million Research/Demonstration
Ethanol Plant Purpose To
demonstrate, evaluate, and improve cellulosic
ethanol processes using
Florida feedstocks To improve efficiency
and decrease process complexity To
facilitate and accelerate commercialization in
Florida
13
Brighter future Renewable Fuels Chemicals
CO2
CO2
CO2

• Displacement of oil
• Recycling waste
• Commodity chemicals
• polylactic acid
• 3-HP, 1-3 PD
• Solvents, acids
• Fuels
• ethanol
• biodiesel
• power
• Rural Employment

• Carbon
• sequestration
• (short term)..

DOE, 2002