EuroFISH: towards standardized translocation detection in pathology

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Euro-FISH towards standardized translocation
detection in pathology
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GoalTo validate a robust, standardized
protocol, throughout Europe and validate the
diagnostic approach on selected entities of
lymphomas
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Rationale

• Lymphoma classification increasingly significant
• Increasing number of specific genetic alterations
• FISH-technology within reach of pathology
  laboratories
• Standardization required

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Euro-FISH

• 9 different laboratories cooperate
• 10 WHO categories
• supplemented with reactive cases.
• 16 different FISH probes have been tested
• Sponsoring by Dako
• Equipment materials
• Meetings
• Training
• Symposium Vienna (EAHP congress Vienna, October
  2006 )
• www.euro-fish.org

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Fluorescence in Situ Hybridization
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Fusion-Signal FISH
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Split-signal FISH
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(No Transcript)
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Large cell lymphoma Case 2
Myc split-apart probe Probe 12
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Large cell lymphoma Case 2
Myc split-apart probe Probe 12
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Results
Stage 1 Robustness of the standardized protocol
90,5 of cases resulted in reliable signals
10 min digestion time proved to be the optimal
time considering morphology and percentage of
reliable signals
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Stage 2 Can the FISH protocol be used throughout
Europe?

• Preliminary data
• 75 of Stage 2 data are analyzed
• 90 of cones show reliable results
• cones without a score (13 out of 144) gt55 no
  score
• corresponds to 80 failure in morphology (in
  more than 35)
• Probe performance
• TCL1, BCL10, MALT, CCDN1 and BCL2 comparable
  duplicates with regard
• to background and signal intensity

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Stage 2 Can the FISH protocol be used throughout
Europe?

• MCL cases t(1114)(q13q32) (cyclin D1 11q13)
  (IGH 14q32)
• 100 of cases (n12) translocation proven with
  CCDN1 and IGH (literature 70-75)
• MALT cases t(1118)(q21q22) (MLT 18q22)
• 40 of cases (n12) translocation proven with
  MLT1 (literature 30)

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University Hospital Nijmegen, The Netherlands Han
van Krieken Patricia van Cleef Anke van Rijk
Dako, Glostrup, Denmark Niels Foged Andreas
Schønau Steen Mathiessen Tim Poulsen Henrik Aspen
Aarhus Sygehus, Denmark Stephen Hamilton
Dutoit Mette Christiansen
John Radcliffe Hospital Oxford, United
Kingdom David Mason Margaret Jones
Hôpital Henri Mondor Paris, France Christiane
Copie Bergman Philippe Gualard Thierry Molina
University of Frankfurt Germany Martin Hansmann
Anja Mottok
Instituto Português de Oncologia Lisbon,
Portugal José Cabecadas Mateus Crespo
Lara Neto
Universita Degli Studi di Siena Siena, Italy
Lorenzo Leoncini Maria Margherita De
Santi Mario Cocco
Centro Nacional Investigaciones
Oncológicas Madrid, Spain Juan Cruz Cigudosa
Juan Fernando Garcia Sergio Hernandez
Hematopathology Dept. Evaggelismos Hospital,
Athens, Greece Dimitra Anagnostou Evi Pouliou
Erasmus Medical Center Rotterdam, The
Netherlands Jacques van Dongen