ASSOCIATED OF INFLAMMATORY MARKERS WITH SUBCLINICAL ATHEROSCLEROSIS IN TYPE 2 DIABETES

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ASSOCIATED OF INFLAMMATORY MARKERS WITH
SUBCLINICAL ATHEROSCLEROSIS IN TYPE 2 DIABETES

• Caparevic Z, Kostic N, Ilic S, Dekleva M, Pencic
  B, Radojkovic I.
• University Clinic for Internal Medicine, CHC"Dr
  Dragiša Mišovic"
• Department of Endocrinology
• School of Medicine, University of BELGRADE,
  SERBIA AND MONTENEGRO

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Background

• Accumulation, aggregation and oxidative
  modification of LDL are believed to play an
  important role in activation of inflammation.
• Oxidized LDL (OxLDL) is associate with the
  proinflammatory C-reactive protein (CRP)
  suggested risk factors for atherosclerosis
  development.
• Increased carotid intima-media thickness (IMK) is
  a surrogate marker of an early atherosclerosis

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Aim

• The aim of this study was to estimate relations
  between oxidized LDL (OxLDL), C-reactive protein
  (CRP) and common carotid intima-media thickness
  (IMT) in type 2 diabetic patients without
  coronary heart disease (CHD).

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Methods Studied patients

• 44 well controlled type 2 diabetic patients
  without CHD.
• The control group was 27 healthy subjects.
• ? None of the patients had a history of
  cerebrovascular or ischemic heart disease.
• ? They were not treated with any other drugs
  except oral antidiabetics.

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Methods-Biochemical measurements

• Lipids profiles were measured by enzymatic
  methods.
• OxLDL was determined by a commercially available
  sandwich ELISA (Mercodia).
• Hs-CRP was measured using hemiluminiscent methods
  (Immulite-DPC).
• HbA1c was measured by immunoturbidometric method
  using commercialy available kit (Roche).
• Glucose were determined by enzymatic methods.
• Common carotid artery intima-media thickness
  (IMT) was measured by ultrasonography (Hewlett
  Packard Image Point).

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Results-Clinical parameters in diabetic patients
and controls
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Results-Clinical parameters in diabetic patients
and controls
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Levels of OxLDL was significantly increased in
diabetic patients compared to those of controls
Plt0.05
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Levels of CRP was significantly increased in
diabetic patients compared to those of controls
Plt0.05
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Correlation analysis revealed a significant
positive correlation between LDL and OxLDL
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Correlation analysis revealed a significant
positive correlation between CRP and OxLDL
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Correlation analysis revealed a significant
positive correlation between TgHDL and OxLDL
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Conclusion

• The results from our study showed that elevated
  levels of circulating oxLDL and CRP are
  associated with an early atherosclerosis
  development in type 2 diabetes.
• The study authors also concluded that CRP and
  OxLDL measurements tended to identify different
  high-risk groups and that screening for both
  markers provided better prognostic information
  than screening for either alone.