Title: Slide 1 Author: Hugh Last modified by: Hugh Created Date: 6/10/2004 6:54:27 PM Document presentation format: On-screen Show Company: University of Cape Town
Does its structure suggest how replication is accomplished? Monomers of DNA = Nucleotides ... structure from Rosalind Franklin's X-ray crystallographic image of ...
E. Unraveling the DNA at one position changes the superhelicity ... A. Gel electrophoresis. i. 1 dimensional. ii. 2 dimensional. B. Density sedimentation ...
... for transcription Chromatin remodelers also play a role in recombination and repair Chromatin Remodeling Complexes PARP1 and the DDB1-2-CUL4A complex are ...
a large multienzyme compound scans the DNA strand for anomalities ... the gap is repaired by DNA polymerase and DNA ligase enzymes. Double strand repair 1/2 ...
Sample combs, around which molten agarose is poured to form sample wells in the gel. ... Pour the warm solution into a mold which fitted with a well - forming comb. ...
Scientists raced to determine the structure of DNA ... Structure of Nucleotides. in DNA. Each nucleotide ... DNA Structure Helps. Explain How it Duplicates ...
Chapter 3 Cutting DNA molecules 1.DNA is easily broken by physical methods 1) Ultrasound 300bp 2) High speed stirring (1500r/min) 8kb 3) DNA is cut randomly in ...
Combine beneficial traits of two or more organisms to create valuable new ... Mix 2 DNAs together and Ligate (DNA Ligase) Transform. Screen for successful event ...
Biotechnology and Recombinant DNA. Terminology. Biotechnology. Genetic engineering ... DNA fingerprinting. Nanotechnology. Bacteria produce tiny pieces of metal ...
ligate foreign DNA and vector. introduce vector into host. screen ... DNA ... DNA Ligase. Intramolecular vs. Intermolecular. Removal of 5'-PO4 Prevents ...
Genes dependent on topoisomerases are regulated on a chromatin level. Next we tried to correlate both transcriptional plasticity and sensitivity to chromatin ...
Look for abnormalities in the DNA sequence to determine ... DNA to be sequenced is synthesized under conditions that ... DNA is synthesized in the presence of ...
A code made up of pairs of bases carried on the DNA molecule. ... DNA is a double helix made from two strands which are joined together by a pair of bases ...
Watson-Crick Model. DNA consists of two nucleotide strands ... Watson-Crick Model. Figure 13.7. Page 221. Click to view animation. DNA close-up animation. ...
This allows DNA to unwind for duplication and transcription (S = sugar; P ... for transcription and replication; unwinding/unpairing must occur so that ...
Denaturation/Renaturation. Denaturation: separation of the 2 strands of the ... Toroid or. spiral. Example: DNA wraps around. histone protein core within nucleosomes; ...
2) palindrome on early side (early palindrome) 3) AT-rich region on ... T unwinds DNA in the early palindrome. As DNA unwinds, RPA binds to single stranded DNA ...
Xenopus cells depleted for Cdc6/18 do not initiate DNA replication. Origin licensing proteins ... at multiple sites on plasmid DNA in Xenopus egg extracts. ...
The binding affinity for the repressor on DNA is determined by the energy ... CRT1 A repressor of transcription whose levels are very tightly controlled. ...
In addition, helicases move along single stranded DNA with a specific polarity; ... 'Sewing machine' model. Types of enzymes at the replication fork ...
Topological Problems in Replication Linear Chromosomes: Telomerase for replication of the ends Topoisomerases to relieve strain of untwisting and supercoiling
Genome Organisation I Bacterial chromosome is a large (4 Mb in E coli) circular molecule Bacterial cells may also contain small circular chromosomes called plasmids ...
Epigenetic regulation during early embryogenesis reprogramming and remodelling Helena Fulkova Istitute of Animal Science, www.vuzv.cz helena.fulkova@vuzv.cz
Laser Light Scattering - Basic ideas what is it? - The experiment how do you do it? - Some examples systems why do it? Double Slit Experiment screen ...
SV mapping. ND spectra. MC simulation. May 01. RWS. ion counter. E1 ... The sensitive volume of the ND is defined by the relative ion collection efficiency map ...
... our odds of getting this hand from a poker deck (52 cards) 1 2 3 ... instead of 52 cards we have N particles. Find a way to Wmax. under sanity constraints: ...
Gene cassettes in integrons. Cre. Dimer reduction in phage P1 plasmids. XerC/D ... Can also catalyze inversion if recognition sites are oriented correctly, but not ...
Essential groups: amino acid side chains, non-covalent intermediates ... of 2-67 in water can produce succinic acid, but only 4%. The highly reactive ...
3D Graphics Projected onto 2D (Don t be Fooled!!!!) T. J. Peters, University of Connecticut www.cse.uconn.edu/~tpeters Outline: Animation & Approximation Role for ...
An average human mitotic chromosome 5 microns in length contains 5 ... Red GNAT HAT domain. Pink MYST homology region; HAT. Light Blue & Green Zn fingers ...
Wann denke ich an Tumor ? Zwei Beispiele aus der Praxis v d M: 63J w 24.11.09 Lumbalgie 30.11.09 Schmerz Unterbauch seit 3 Tagen in 8 Wo 10 kg Verlust; Sono Gyn: Fl ss.
The inset shows the coordination of a zinc atom by characteristically spaced ... such as phosphatidylinositol-4,5-bisphosphate, PI-3, 4-P2 or PI-3,4,5-P3. ...
Lecture 11 Test next week in class Protein structure Collagen Most abundant protein of vertebrates. Extracellular protein-insoluble fibers, great tensile strength ...
Plasmids mitochrondrial DNA chromosome. E. coli Chromosome ... decatenate and catenate. Catenated (linked) A Review of the Different Topoisomerases ...