PNA panning 75% 1% 6% 81% 0.2% Immunostaining of cells Stained cells after PNA panning 2% Step1. Crosslink proteins to the DNA in each DP cell with formaldehyde Double-
Identifying Transcription Factors that Bind the Cd4 Promoter Matthew C. Surdel and Sophia D. Sarafova Davidson College Biology Department I. Background and Observations
This research was made possible by Merck/AAAS Biochemistry Internship Program. ... Analytical biochemistry. 290, 147-178. Sarafova, S., Siu, G. 1999. ...
Both are classified as T-cells and derive from a common precursor, but only CD4 ... Methods in Enzymology. 254: 595-604. Figure 2 (Sarafova and Siu, 2000) ...
and flowcytometry of fresh B10.A thymocytes 84.37% Thymus cells 6.91% B Two color immunostaining and flowcytometry of PNA-panned B10.A thymocytes PNA Cell Panning PNA
Determining the Existence of a Second Promoter in the CD4 ... and save the supernatant. bead. TBP (save) bead. Step 3. Add pre-blocked beads and TBP antibody. ...