HPTS-%20Optical%20pH%20Sensor

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HPTS- Optical pH Sensor

• pH sensitive fluorescent dye
• Absorbance / Excitation changes with pH
• Emission at 520 nm
• Excitation Ratiometric

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pH SENSOR ASSEMBLY FOROPTICAL CHEMICAL SENSOR
White microfiltration membrane backing
PEG-dye copolymer hydrogel
Transfer adhesive
Note All Optical Chemical Sensors Are Based on
Same Concept as pH Sensor
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pH Sensor Calibration in Cell Suspension
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E.coli Shake Flask Fermentation with pH Coaster
and Sensor Patch

• Conditions LB Media, T37C, 300 rpm
• Discrepancy at 6 hrs likely due to CO2

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Measurement of pCO2

• Important metabolite
• Critical in bacterial and mammalian cell cultures
• Conventional technology uses pH probe inside
  bicarbonate buffer- cumbersome
• New optical alternative

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DISSOLVED OXYGEN OPTICAL-CHEMICAL SENSOR
CONSTRUCTION
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On-line turbidity measurement in E.coli
fermentation