Comparing CP and NCP BVDV Vaccines: Antigen Trafficking Increased to Mucosal Surfaces with NCP Vaccines

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Comparing CP and NCP BVDV Vaccines Antigen
Trafficking Increased to Mucosal Surfaces with
NCP Vaccines

• CCL Chase1, LJ Braun1, L Holler1 and M Spire2
• 1Dept Vet Sci, SDSU, Brookings, SD
  2Schering-Plough Animal Health, Elkhorn NE

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Background

• Distribution of CP NCP BVDV Spagnulo-Weaver et
  al 1997
• Infected with MD case (NCP or CP) or cloned NCP
  or CP- found NCP longer and in more tissues
• Biotypes- Lambot et al 1997 1998
• Immune Response Difference between NCP or CP pair
• Faster with NCP and decreased virus shed

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Materials Methods

• 24 Non-suckled calves 3-4 weeks of age (CD)
• Colostral whey product BVDV antibody negative

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Materials Methods
Vaccinated IM in the left side of the
neck Euthanized calves at 0, 3, 6, 8, 10, 12, 15,
or 18 days
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Materials Methods
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Materials Methods

• Immunohistochemistry on the tissues using 15C5
  mAb
• Pathologist was blinded to calves and tissue
  origin
• Positive tissues were graded on a scale of 1-4,
• with 4 demonstrating the most severe BVDV
  infection.
• Each set of tissues was from 6 animals so the
  highest
• possible score would be 24 (444444).

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Results
Positive Control PI
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Results
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Results
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Results
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Results
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Results
Group 1 Day 8
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Results
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Results
Group 2 Day 8
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Results
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Results
Group 3
Day 8
Day 15
Lymphoid depletion at day 8- Repopulated by day 15
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Summary

• Antigen from a NCP vaccines localized in the
  Peyers Patches at day 6 and was sustained to day
  18 day.
• Antigen from CP vaccine varied by isolate- one
  vaccine had one day positive another 3 days
• Implications- mucosal immunity

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