Transgenesis

1

• Transgenesis

2
Figure 20-22
Methods of introducing a transgene
3
Creation of Mus musculus transgenes
4
Figure 20-31a
Producing cells containing a targeted gene
knockout
5
Figure 20-29
Creation of Drosophila melanogaster transgenes
6
(No Transcript)
7
(No Transcript)
8
(No Transcript)
9
(No Transcript)
10
P element
piggyBac
11
Insect transposon vectors
12
Host range of transposons
Mosquito Silkmoth
Flour Beetle
13
Transformation of Planaria (roundworm)
14
(No Transcript)
15
(No Transcript)
16
(No Transcript)
17
Potential for broad host range transposons in
mutagenesis

• Insertional mutagenesis (random)
• Transgenic RNAi
• Homologous recombination? (a la Drosophila)

18
Infection by the Ti plasmid
19
Figure 20-25
The Ti plasmid of Agrobacterium tumefaciens
20
The generation of a transgenic plant
21
Pattern of transmission of T-DNA
22
(No Transcript)
23
(No Transcript)
24
(No Transcript)
25
(No Transcript)
26
(No Transcript)
27
(No Transcript)