MIAHA - Minimum Information for a High Content Assay - a proposal for a standard for high content analysis data interchange

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MIAHA - Minimum Information for a High Content
Assay - a proposal for a standard for high
content analysis data interchange

• Mark A Collins Ph.D
• Marketing Manager, Cellular Imaging Analysis

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Review

• Standard for Images and Data
• An image standard alone is not enough
• To analyse an image between different packages
  need more than just its type and format
• How acquired
• Magnification
• Filters etc
• Need a standard for data interchange that uses
  appropriate meta data to describe HCS/HCA images
  and data
• Self describing format of XML proposed
• Embrace and extend two existing standards
• OME Open Microscopy Environment
• http//www.openmicroscopy.org/
• MIACA Minimum Information for A Cellular Assay
• http//miaca.sourceforge.net/

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Practical Implementation of a Standard

• Not just talk
• Draft specification for HCS/HCA Data Interchange
  completed
• MIAHA
• Minimum Information for A High Content Assay
• Testing the standard
• HCSGateway Cellomics open interface to third
  party readers
• GE INCELL 1000/3000
• Evotec Opera
• Implemented the standard as part of the
  HCSGateway product
• Get third party data in to our informatics
• Get third party data out

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Implementing a Standard Generation 1
HCSGateway transform to MIAHA
3rd party Analysis software
Instrument data Images
MIAHA XML Document image links
Webservices
Webservices API natively reads and writes
MIAHA XML standard
Cellomics Store database
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Implementing a standard - Generation 2
Instrument/Analysis software directly
writes/reads MIAHA XML data
MIAHA XML Document image links
Webservices
Webservices API natively reads and writes
MIAHA XML standard
Cellomics Store database
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Current Standard Usage

• Generation 2 Implementation
• Evotec Opera
• GeneData Screener
• ArrayScan
• CellWoRx
• Generation 1 Implementation
• INCELL 1000
• INCELL 3000
• Other custom

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HCSGateway Open Instrument interface -
implements MIAHA
Cellomics Reader Family
GE IN Cell Analyzer 1000
API CellWoRx Reader
HCSGateway Open Interface for Instrumentation
GE IN Cell Analyzer 3000
Other Instruments
Evotec Opera