COMPARISON OF BIOAVAILABILITY OF

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• COMPARISON OF BIOAVAILABILITY OF
• DIFFERENT FORMS OF CURCUMIN USING
• NON-EVERTED RAT INTESTINAL SAC MODEL

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• Turmeric is dried yellow powder obtained
  
  from the rhizomes of
  Curcuma longa Family Zingiberaceae
• Curcumin is the most plentiful of the
  curcuminoids found in turmeric
• Biocurcumax is a patented extract of the rhizome
  of Curcuma longa, standardised to provide 95 of
  natural mixed curcuminoids
• and sesquiterpenoids
• Curcumin is consumed as turmeric in many forms
  either as a spice in our daily meals or
• as a traditional remedy

Turmeric Rhizomes
Curcuma longa Plant
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CURCUMIN

1, 7-bis (4-hydroxy-3-methoxyphenyl) -1,
6-heptadiene-3, 5- dione

• Molecular formula C21H20O6
• Molar mass 368.38 g/mol
• Curcumin is, yellow pigment, active component of
  turmeric
• It is orange-yellow crystalline powder,
  insoluble in water
• Also called diferuloyl methane

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• Physicochemical properties
• Practically insoluble in water
• Log P 2.05
• Undergoes hydrolytic degradation at alkaline pH
• Unstable in presence of light
• Curcumin is unstable in the gut and the traces
  that pass
• through the GI tract rapidly degrades or is
  conjugated
• through glucuronidation
• Ingestion of 2 to 10 grams of curcumin resulted
  in
• undetectable to very low serum levels
• Limited clinical application due to poor aqueous
• solubility, poor absorption and extensive
  metabolism
• leading to decreased bioavailability

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• As spices and colorant
• As cosmetic
• As Blood purifier
• As a remedy for ulcer
• Common cold, wound,
• Sore throat, leprosy
• inflammation etc.

• Anticancer activity
• Antioxidant activity
• Antidiabetic activity
• Antirheumatic activity
• Antimicrobial activity
• Hepatoprotective and
• Nephroprotective activity
  
  

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• Comparison of bioavailability of different
  
  forms of curcumin
  using non-everted
• rat intestinal sac model

BIOCURCUMAX
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BIOAVAILABILITY/PERMEATION FROM G.I.T
PURE CURCUMIN
TURMERIC
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• Male wistar rats (weighing 250-300 g) were used
  for the study
• The clean intestinal tract was prepared into 8
  0.2 cm long sacs having a diameter of 3 0.5 mm
• Each sac was filled with 0.5 ml of drug
  suspension and tied with thread to a length of
  approximately 5 cm
• Each non-everted intestinal sac was placed in a
  glass conical flask containing KRPBS (50 ml) with
  provision for aeration
• The sacs were maintained at 37 C in a shaking
  water bath operating at 50 strokes per min
• Samples were withdrawn from outside the sacs
  every 30 min for
• 8 h and replaced with fresh buffer
• The samples were analyzed spectrophotometrically
  at ?max 422nm

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Graphs showing Permeation in 8
h from different vehicles
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COMPARITIVE PERMEATION PROFILE OF DIFFERENT FORMS
OF CURCUMIN FROM DIFFERENT VEHICLES
Results of the study indicate that -
Permeation in 8 h from the rat intestine was
maximum for Corn oil
from all the forms of curcumin - The pattern
of Permeation profile for different forms of
curcumin from Corn oil is
Biocurcumax(26.3) gt Turmeric(19.9)gt
Pure curcumin(13.4) - The pattern for
Permeation profile from different vehicles is
Corn oil(26.3)gt R.Butter(16.9)gt
Milk(6.8)gt Aq. Suspension(2.5)
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curcumin
turmeric

The Present study concludes that -
Permeation of curcumin increase as the
lipophilicity of the vehicle
increases - Permeation is maximum from
Biocurcumax followed by

Turmeric and Pure curcumin - Curcumin
should be consumed with oily vehicles instead
of water as Biocurcumax or Turmeric
for its maximum therapeutic effects
REFERENCES 1. Hatcher H, Planalp R, Cho J, Torti
FM, Torti SV, Curcumin from ancient medicine to
current clinical trials, Cell. Mol. Life Sci.,
2008, 65 (11) 163152 2. Ruan LP, Chen S, Yu BY,
Zhu DN, Cordell GA, Qiu SX, Prediction of human
absorption of natural compounds by the
non-everted rat intestinal sac model, Eur. J.
Med. Chem., 2006, 41 605-610