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Ribosomes

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check for correct codon recognition. Spezielle Genetik und Molekularbiologie SS 2006 ... EF-Tu GTP hydrolysis in codon recognition. Mol. Biol. of the Gene ... – PowerPoint PPT presentation

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Title: Ribosomes


1
Ribosomes
Mol. Biol. of the Cell
2
Prokaryotic ribosome
Mol. Biol. of the Gene
3
Eukaroytic ribosome
Mol. Biol. of the Gene
4
Sedimentation of ribosomes
Mol. Biol. of the Gene
5
The ribosome cycle
Mol. Biol. of the Gene
6
Polyribosomes (polysome)
Mol. Biol. of the Gene
7
Peptidyl transferase reaction
Mol. Biol. of the Gene
8
tRNA binding sites
Mol. Biol. of the Gene
9
Overview on translation initiation
Mol. Biol. of the Gene
10
Ribosome binding site (Shine-Dalgarno sequence)
on mRNA
Mol. Biol. of the Gene
11
Internal start site are important for
polycistronic mRNAs
Mol. Biol. of the Cell
12
N-formyl methionine
Mol. Biol. of the Gene
13
Initiation factors bound to the ribosome
Mol. Biol. of the Gene
14
Sequence of events during initiation in
prokaryotes
Mol. Biol. of the Gene
15
Capping
Mol. Biol. of the Gene
16
Translation initiation in eukaryotes
Mol. Biol. of the Gene
17
Start codon identification in eukaryotes
Kozak sequence at start codon G/ANNAUGG
Mol. Biol. of the Gene
18
Circular polysomes in eukaryotes
Mol. Biol. of the Gene
19
Circular polysomes
Mol. Biol. of the Cell
20
Short upstream ORFs in eukaryotes
Mol. Biol. of the Gene
21
Internal ribosome entry sites in eukaryotes (IRES)
Mol. Biol. of the Gene
22
Overview of the elongation phase
Mol. Biol. of the Gene
23
Binding of aa-tRNA to the A-site
EF-Tu protection of ester from hydrolysis check
for correct codon recognition
Mol. Biol. of the Gene
24
Codon recognition by the anticodon
Mol. Biol. of the Gene
25
EF-Tu GTP hydrolysis in codon recognition
Mol. Biol. of the Gene
26
Accommodation as the second recognition control
step
Mol. Biol. of the Gene
27
Translocation
Mol. Biol. of the Gene
28
Release factors
Class I (stop codon recognition) prokaryotic RF1
UAG, UAA RF2 UGA, UAA eukaryotic eRF1 UAA,
UAG, UGA Class II (diss. of class I
factors) RF3 eRF3
Mol. Biol. of the Gene
29
Action of release factors
Mol. Biol. of the Gene
30
Ribosome recycling factor
Mol. Biol. of the Gene
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