Richard Owen, 1843

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Richard Owen, 1843 defined concept of homologous
organs Homeotic transformations of organs in
plants Metamorphosis of Plants Published by
Johan Goethe, 1790
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Wild type.                APETALA1   
PISTILLATA    AGAMOUS
                                 "A"
class           "B" class         
"C" class
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Wild type.                APETALA1   
PISTILLATA    AGAMOUS
                                 "A"
class           "B" class         
"C" class
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AP1 mRNA
AG1 mRNA
AP1 in AG1 mutant
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550 MYA
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plena
Shp1/2
Plena clade
Ag Far
AG clade
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Central zone
adaxial
abaxial
apical
basal
phyllotaxy
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Abaxial domain
Adaxial domain
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adaxial
abaxial
Lateral primordia formation/polarity
abaxial identity crabsclaw, YABBY, KANADI -
arabidopsis adaxial identity phantastica -
Antirrhinum roughsheath - maize phabulosa -
arabidopsis
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McConnell and Barton. 1998. Development 1252935
1. Ad/ab polarity is needed for lateral
expansion
2. Adaxial cells determine initiation of the
axial meristem
Wild type
Phb
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Wild Type
phabulosa
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Dominant Phabulosa/Phavulota mutations
START Sterol binding domain
HomeoBox- Zipper DNA binding domain
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WT
Phb
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Triple knockout phenotype
Emery et al. 2003. Current Biology, Vol. 13,
17681774,
Dominant Phb phenotype
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Boundary required for Later growth of leaf blade
Abaxial domain
Adaxial domain
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Abaxial determinants
Development 126, 4117-4128 (1999) Members of the
YABBYgene family specify abaxial cell fate in
Arabidopsis Kellee R. Siegfried1,, Yuval Eshed1,
Stuart F. Baum1, Denichiro Otsuga2, Gary N.
Drews2 and John L. Bowman1,
KANADI regulates organ polarity in
Arabidopsis Randall A. Kerstetter, Krista
Bollman, R. Alexandra Taylor, Kirsten Bomblies
R. Scott Poethig 2001 Nature 411706-8
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Dominant Phabulosa/Phavulota mutations
START Sterol binding domain
HomeoBox- Zipper DNA binding domain
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Carrington and Ambros 2003, Science 301337
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PTGS pathway in eukaryotes
Carpel Factory (DCL1)
Adapted from Williams and Rubin 2001, PNAS
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Jacobsen et al. 1998. Development 1265231
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Kidner and Martienssen, Nature. 2004 Mar
4428(6978)81-4
Argonaut phenotypes AGO is a component of the
RISC RNA induced silencing complex
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PHB WT
miR165 WT
miR165 ago
PHB ago
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Boundary required for Later growth of leaf blade
Abaxial domain
Adaxial domain
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Asymmetric expression of YAB3 requires KANADI
function
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PHABULOSA expression in the radialized (adaxialize
d) KANADI triple mutant
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In tomato, YABBY is restricted to cytoplasmic
dense cells at the margins of the ad/ab boundary
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Emery et al. 2003. Current Biology, Vol. 13,
17681774,
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Bohmert et al EMBO J 17170-180
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Meristem initiation/maintenance
Knotted -maize shoot apical meristemless -arabid
opsis
Meristem size
clavata1 clavata2 clavata3
Lateral organ polarity
Abaxial genes crabsclaw, YABBY Kanadi Adaxial
genes phabulosa Phavoluta Revoluta
Organ number and polarity Carpel
Factory Argonaute
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Dominant Phabulosa/Phavulota mutations
START Sterol binding domain
HomeoBox- Zipper DNA binding domain
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Bohmert et al EMBO J 17170-180
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adaxial
abaxial
Lateral primordia formation/polarity
abaxial identity crabsclaw, YABBY, KANADI -
arabidopsis adaxial identity phantastica -
Antirrhinum roughsheath - maize phabulosa -
arabidopsis
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Carrington and Ambros 2003, Science 301337
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(No Transcript)
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Wild type.                APETALA1   
PISTILLATA    AGAMOUS
                                 "A"
class           "B" class         
"C" class
53
AP1 mRNA
AG1 mRNA
AP1 in AG1 mutant
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Dominant Phabulosa/Phavulota mutations
START Sterol binding domain
HomeoBox- Zipper DNA binding domain
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Phv
Adaxial domain
Domain boundary Required for lateral growth
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WT
clv3
clv3
Fletcher et al. 1999. Science 2831911-14
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KANADI expression in the abaxial domain
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Development 126, 4117-4128 (1999) Members of the
YABBYgene family specify abaxial cell fate in
Arabidopsis Kellee R. Siegfried1,, Yuval Eshed1,
Stuart F. Baum1, Denichiro Otsuga2, Gary N.
Drews2 and John L. Bowman1,
61
Development 126, 4117-4128 (1999) Members of the
YABBYgene family specify abaxial cell fate in
Arabidopsis Kellee R. Siegfried1,, Yuval Eshed1,
Stuart F. Baum1, Denichiro Otsuga2, Gary N.
Drews2 and John L. Bowman1,
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Current Biology 2001, 1112511260
Establishment of polarity in lateral organs of
plants Yuval Eshed, Stuart F. Baum, John V.
Perea and John L. Bowman
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