Wireless Monitoring of Cell Cultures in Incubation

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Wireless Monitoring of Cell Cultures in Incubation

• Group 2
• Karl Zelik
• David Ding
• Carrie Biederstadt
• Mentor Dr. Igor Efimov

2
Background

• Cell cultures are used for a variety of different
  types of research
• Cultures must be incubated in sterile
  environments
• Due to regular handling by researchers, cultures
  are very susceptible to contamination
• Researchers lose money and time when cultures
  become contaminated

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Purpose

• Reduce likelihood of contamination by reducing
  contact between researcher and culture
• Design wireless system capable of monitoring
  Petri Dish
• Temperature
• pH
• Oxygen Concentration
• The System should also be capable of
• Electrical Stimulation Recording
• Photofluorescent Measurement

4
Design Requirements

• Battery Life 2-3 weeks
• Range 10ft
• Accuracy of Measurements
• Temperature 1 C (from 0-40 C)
• pH 0.2 (from 0-14)
• Oxygen 0.2 (from 0 to 100 saturation)

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Overview of Chosen Design Concept

• Petri Dish will have an internal well.
• The electronics will be around the well on the
  inside of the Petri dish.
• Electrode Matrix will be on the bottom.
• Integrated internal and independent external RF
  receivers.
• Data will be stored in an external computer.

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Calculations for Wireless Range
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Petri Dish Design
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Incubator

• Sanyo MCO-20AIC
• 5795.00
• 5 weeks lead time
• .7m x .75m x .9m

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System on Chip

• 2.4 GHz Radio Frequency Transceiver/Microcontrolle
  r
• Chipcon CC2510
• 3.00
• 14 bit ADC with 8 inputs
• 12 week lead time
• Current consumption about 24mA (running at 26
  MHz)
• On chip temperature sensor (accuracy of 1 C)

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Petri Dish

• 100mm with 40mm well
• 5mm deep well
• Clear Fused Quartz made by Technical Glass
  Products
• 225
• 4 weeks lead time

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Sensors

• pH
• Sentron custom made design similar to their MICRO
  electrode.
• 8mm x 3mm
• 0-14 pH at -5C to 75C accuracy of .2
• Under 3V power supply
• Waiting on price quote
• Oxygen Concentration
• SMSI Oxygen Sensor
• 9mm x 5mm x 4.5mm
• 1mA input current
• 0-100 saturation at an accuracy of .2
• Waiting on price quote

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Electrodes

• 4 Indium Tin-oxide Transparent Film Electrode
• Bekaert Specialty Films
• Custom Design
• Waiting on quote

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Data Transmission

• Antenna
• Fractus FR05-S1-N-0-102
• 5.00
• 2.4-2.5 GHz Range
• 1 dBi peak gain

14
Photofluorescence

• Photodiode
• Hamamatsu Si photodiode S2386-18
• 5.4mm x 5.4mm x 3.6mm
• Dark Current 2pA
• 4
• LED
• Mini-molded Rohm Electronics surface mount LED
  SML-512 (wavelength)
• 1.6mm x .8mm x .4mm
• 20-25mA current supply
• 0.18

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Battery

• Panasonic CR2477
• 2.85
• 24.5mm x 7.7mm
• 1000mAh

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Circuit Diagram
On Petri Dish
On Microcontroller
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Software Development

• The software kit, Chipcon CC2500/CC2550DK, will
  program the MCU and transceiver.
• Smart RF studio is a free software package from
  Chipcon that configures the wireless network.

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Overview of Manufacturing Processes

• Ayanda Biosystems will manufacture the
  transparent chip where the sensors will be
  embedded.
• PC Board Fabrication
• PCB Express
• Sanyo will build the transceiver into their
  incubator system.

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Design Safe

• There are no safety issues besides working with
  the cell cultures.
• Possible safety issue when maintenance performed.

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Conclusions

• All of our requirements were met.
• Our design monitors, pH, oxygen concentration,
  and temperature
• The cells can be stimulated electrically and by
  photofluorescence
• Our system is wireless and the cells are able to
  be viewed under the microscope without an
  interruption of data transmission
• Even though cost of the products are inexpensive,
  the manufacturing costs will be high.
• Patentable.

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Future Considerations

• We would like to use a smaller Petri dish in the
  future as it is more readily available and
  cheaper.
• As more sensing technology becomes available, we
  can add it to our dish.

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Lessons

• It is hard to find exactly what you need online.
• Need to give companies a lot of time to respond
  to your quote inquiries.
• The design process is dynamic.

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References

• http//www.hpk.co.jp/Eng/products/ssd/pdf/s2386_se
  ries_kspd1035e02.pdf
• http//www.rohm.com/products/databook/led/pdf/sml-
  51.pdf
• http//www.excesssolutions.com/cgi-bin/item/ES2334
  
• http//chipcon.com/
• http//www.s4ms.com/oxygen_general.htm
• http//sanyo.com/
• http//www.sentron.nl/nieuw/index.php?id139

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Questions???

• Website
• http//userfs.cec.wustl.edu/kez1/petri/petri.html