Expression of Specific chemokines and chemokine receptors in the central nervous system of multiple

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Expression of Specific chemokines and chemokine
receptors in the central nervous system of
multiple sclerosis patientsByEsther Valbrun

• Sorensen, TL et al. 1999. Expression of specific
  chemokines and chemokine receptors in the central
  nervous system of multiple sclerosis patients. J
  Clin Invest. 103(6)807-15.

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Introduction

• Multiple Sclerosis (MS)
• Central Nervous System (CNS)
• Chemokine Receptor
• Chemokine
• RANTES

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Investigation

• Find expression of chemokines in CNS
• Expression by infiltrating cells

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Graphical Analysis of IP-10 concentration and
leukocyte count

• Regression analysis
• - indicated a significant linear relationship
  between CSF cell count and IP-10 concentration

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IP-10 Concentration vs Leukocyte Count
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Inflammatory demyelination in an active MS lesion
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Analysis Expression of CXCR3 and IP-10

• Demyelinating MS brain lesions
• - analyzed with anti-CXCR3 antibodies
• Identified CXCR3-positive cells

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Expression of CXCR3 and IP-10 in MS lesions
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Studies

• CXCR3-positive CD4 T cells
• - supported the functional significance of
  IP-10/MigCXCR3 interactions in recruiting CD4 T
  cells
• CCR5-positive T cells

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CXCR3 and CCR5

• IP-10/MigCXCR3 and RANTESCCR5
• CSF chemokine levels are altered during attacks
  of symptomatic inflammatory demyelination
• Activated T cells expressing CXCR3
• 80 of CD4 cells in MS CSF expressed CXCR3
• CCR5 was expressed

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Conclusion

• Found consistent expression
• - chemokines and chemokine receptors during
  active MS
• Increased accumulation
• - CXCR3-positive and CCR5-positive CSF cells
• Supported by the uniform detection
• - CXCR3 and CCR5

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Reference

• Dictionary of Cell and Molecular Biology
• Sorensen, TL et al. 1999. Expression of specific
  chemokines and chemokine receptors in the central
  nervous system of multiple sclerosis patients. J
  Clin Invest. 103(6)807-15