Determination of Calcium and Magnesium in 2% milk vs' Skim Milk

1
Determination of Calcium and Magnesium in 2 milk
vs. Skim Milk

• By Meghana Mehta
• 2 clover green spring clover green
• reduced fat milk spring skim milk

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Background info on calcium and magnesium

• CALCIUM
• essential to proper formation of bone and teeth
• adult RDA is 1000mg/day

• MAGNESIUM
• regulates nerve and muscle cell functions
• adult RDA is 300-350 mg/day

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Magnesium Deficiency Symptoms
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Calcium Deficiency Symptoms

• Bone thinning
• Osteomalacia-reduction of mineralization of bone

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Goal of experiment?

• To use EDTA titration method to compare the
  amount of calcium and magnesium in 2 milk vs.
  skim milk!
• To find out if the daily allowance of Ca in
  skim and 2 milk matches the amount found
  experimentally!

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How do EDTA titrations work?

• EDTA is a titrant that can be used
• It is a hexaprotic ion
• Works by binding to metal ions
• HO2CCH2 CH2CO2H
• HNCH2CH2NH
• HO2CCH2 CH2CO2H
• 2 REACTIONS.
• Ca2 EDTA CaEDTA4-
• -Magnesium works the same way

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Hypothesis??

• 1.That there is the same amount of calcium and
  magnesium in both 2 and skim milk
• 2. The daily value in skim and 2 milk should
  correspond after doing calculations
• Chart shows Daily value on the back of the milk
  carton

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Procedure as follows

• For calcium determination
• made calcium standard using CaCO3
• standardized EDTA solution against it
• performed titrations with calcium standard and
  Hydroxynapthol blue as indicator
• blue violet
• Magnesium and calcium determination
• made standard EDTA solution
• titrated milk samples with EDTA and Erichrome
  Black T indicator
• wine red blue

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Results Molarities of 2 types of milk

• Skim milk 2 milk
• .04180 M Ca2 .03931 M Ca2
• .03412 M Ca2 Mg2 .03404 M Ca2
  Mg2

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Av. for 2 MILK 37.85Do known values
correspond to ones found experimentallyAv.
for SKIM MILK 40.25
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Conclusion

• Both 2 and skim milk have roughly the same
  amount of calcium
• Both have the same amount of total calcium and
  magnesium
• they are both just as good to drink
• the daily values match up within 10-explained
  by experimental error perhaps

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OTHER METHODS

• Ion selective electrode
• another way to measure ion concentration
• most widely used analytical technique
• fast and effective
• Stuck to one method rather than 2!

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DRINK MILK.IT DOES A BODY GOOD!!!
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References

• Harris C. Daniel. Quantitative Chemical
  Analysis-5th edition. NY WH Freeman co. 1999
  p.310-311
• lthttp//www.whymilk.com/about/nutrient/index.htmlgt
  
• lthttp//www.bodybuilding.com/store/cmz.htmlgt
• lthttp//www.notmilk.comgt
• lthttp//www.gotmilk.comgt
• lthttp//www.milk.comgt
• McCormick, G. Patrick. Titration of Calcium and
  Magnesium in Milk with EDTA Journal of
  Chemical Education p.136-139
• lthttp//www.orionres.com/labcat/ise/iseindex.htmlgt