Diagnosis of Aspergillosis by Antigen Detection

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Diagnosis of Aspergillosis by Antigen Detection

• L. Joseph Wheat Chadi Hage
• MiraVista Diagnostics
• Indiana University Medical Center

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Airborne Conidida Infectious
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Angio-Invasive ?Dissemination
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CT Findings
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Halo Sign
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Evolution of CT Findings
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Air Crescent
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Aspergillosis in Non-immunocompromised Host

• Fungus Ball

• Chronic Invasive

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Difficulty in Diagnosis
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Platelia Aspergillus EIA

• Platelia EIA

• Example

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EDTA-Heat Pre-treatment
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Dissociation Immune Complexes
EDTA
100 C
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Need for EDTA-Heat Pretreatment
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Bronchopulmonary Lavage
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Analysis of Proven or ProbablePossible Excluded
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Hematology
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Bone Marrow Transplant
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Hematology
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Lung Transplant Surveillance
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Solid Organ Transplant
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GM Index inSolid Organ Transplant
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Intensive Care Unit
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Non-Immunocompromised
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Summary Platelia BAL for Diagnosis of IPA
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Experience in Clinical Testing
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Causes for False Results
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False-positive Aspergillus Antigenemia in
Histoplasmosis
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False-Positive Platelia in Histoplasmosis
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Sensitivity for Detection of Purified
Galactomannan
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Consequence of False-Positive in Histoplasmosis
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Diagnostic Approach in IPA