Introduction to ELISA

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Introduction to ELISA
ELISA short for enzyme-linked immunospecific
assay A technique that measures the amount of a
specific protein in a solution
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The Use of Assays

• If a substance is chosen as a substance of
  interest, researchers and manufacturing must be
  able to test for its presence, activity, and
  concentration. The product must be assayed.
• Other assays are done for potency, toxicity, and
  stability.
• Assays are performed at every step in the
  research, development, manufacture, and quality
  control of a product.

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The 24-well plate reveals samples with different
amounts of amylase activity. Amylase breaks down
starch to sugar. In the activity assay, as
amylase breaks down starch, the dark color of a
starch/iodine mixture becomes lighter. The
lighter the color, the more active the amylase.
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ELISA
ELISA use an antibody to recognize a specific
protein (Ag). An enzyme bound to the Ab causes
a color change in a substrate. The more color
in an ELISA, the more Ab-enzyme complex is bound
to target protein, indicating a higher Ab
present.
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From the Course Planner
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