Identify Patient Zero of a Zombie Apocalypse With the Power of an ELISA!

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• Identify Patient Zero of a Zombie Apocalypse With
  the Power of an ELISA!

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Bio-Rad Curriculum and Training Specialist
Tamica Stubbs tamica_stubbs_at_bio-rad.com
Damon Tighe damon_tighe_at_bio-rad.com
Sherri Andrews, Ph.D. sherri_andrews_at_bio-rad.com
Leigh Brown leigh_brown_at_bio-rad.com
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TV or real life?
The Walking Dead show
Feb 11,2014 Atlanta
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Zombiefication

• Method of infection
• Bodily fluid contact
• Airborne
• Foodborne
• Symptoms
• Undead
• Hungry for flesh
• Treatment
• None known

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• Introduction to Immunology, Antibodies and ELISA
• Fluid swapping and disease spreading!
• Indirect ELISA to narrow down who were the
  initial carriers of the disease

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Crash Course in Immunology and ELISA
Immune Response
C. Macrophage
A. Pathogen
Zombie virus
D. Macrophage
B. B cells
E. Macrophage
F. T cell
G. B cell
J. Antibodies attach to pathogen
Antibodies are ineffective Against Zombie Virus
H. Memory B cells
I. Plasma cells
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Crash Course in Immunology and ELISA
Antibody Structure
ELISA tests are based on immune system antibody
molecules
Antigens
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ELISA Enzyme-Linked Immunosorbant Assay
(indirect)
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Swapping Fluids and Disease
Swap with 1 person at your table (record who)
Add your 750 µl together, mix and each take 750
µl back
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Swapping Fluids and Disease
Swap with 1 person at the closest table (record
who)
Add your 750 µl together, mix and each take 750
µl back
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Swapping Fluids and Disease
Swap with 1 person at the another table (record
who)
Add your 750 µl together, mix and each take 750
µl back
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ELISA Time! Testing for who is infected
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ELISA Time!Testing for who is infected
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ELISA Time!Testing for who is infected
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Add 50ul - (wash buffer)
Your ID
Your ID
Your ID
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Add 50ul Bodily Fluid
Your ID
Your ID
Your ID
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ELISA Time! Testing for who is infected
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ELISA Time!Testing for who is infected
Concept Corner
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ELISA Time!Testing for who is infected
Concept Corner
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ELISA Time!Testing for who is infected
Concept Corner
Hydrophobic interactions
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ELISA Time!Testing for who is infected
Tap out liquid
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ELISA Time!Testing for who is infected
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ELISA Time!Testing for who is infected
Tap out liquid
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ELISA Time!Testing for who is infected
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ELISA Time!Testing for who is infected
Tap out liquid
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ELISA Time!Testing for who is infected
Add 50 µl PA (Primary Antibody)
Your ID
Your ID
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ELISA Time!Testing for who is infected
Incubate 5 minutes
Your ID
Your ID
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ELISA Time!Testing for who is infected
Concept Corner
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ELISA Time!Testing for who is infected
Concept Corner
Your ID
Your ID
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ELISA Time!Testing for who is infected
Concept Corner
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ELISA Time!Testing for who is infected
Tap out liquid
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ELISA Time!Testing for who is infected
Wash with Buffer
Your ID
Your ID
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ELISA Time!Testing for who is infected
Tap out liquid
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ELISA Time!Testing for who is infected
Wash with Buffer
Your ID
Your ID
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ELISA Time!Testing for who is infected
Tap out liquid
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ELISA Time!Testing for who is infected
Add 50ul SA (Secondary Antibody)
Your ID
Your ID
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ELISA Time!Testing for who is infected
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ELISA Time!Testing for who is infected
Concept Corner
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ELISA Time!Testing for who is infected
Concept Corner
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ELISA Time!Testing for who is infected
Concept Corner
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ELISA Time!Testing for who is infected
Concept Corner
Antigen chicken gamma globulin
Primary Antibody anti-chicken
polyclonal antibody
Secondary Antibody goat anti-rabbit conjugate
to HRP
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ELISA Time!Testing for who is infected
Tap out liquid
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ELISA Time!Testing for who is infected
Wash with Buffer
Your ID
Your ID
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ELISA Time!Testing for who is infected
Tap out liquid
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ELISA Time!Testing for who is infected
Wash with Buffer
Your ID
Your ID
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ELISA Time!Testing for who is infected
Tap out liquid
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ELISA Time!Testing for who is infected
Wash with Buffer
Your ID
Your ID
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ELISA Time!Testing for who is infected
Tap out liquid
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ELISA Time!Testing for who is infected
Add 50 µl Sub (HRP substrate)
Your ID
Your ID
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ELISA Time!Testing for who is infected
Incubate 5 minutes Watch for color change!
Your ID
Your ID
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ELISA Time!Testing for who is infected
Concept Corner
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ELISA Time!Testing for who is infected
Concept Corner
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ELISA Time!Testing for who is infected
Concept Corner
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Deducing who was originally infected
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Deducing who was originally infected

• Everyone Stand UP!
• If your ELISA was negative sit down

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Deducing who was originally infected

• Round 1
• If someone you shared with just sat down, also
  sit down
• Round 2
• If someone you shared with just sat down, also
  sit down
• Round 3
• If someone you shared with just sat down, also
  sit down

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Deducing who was originally infected
Who is left?
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What did we use today?
Bio-Rads Immuno Explorer Lab
166-2400EDU
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Other Zombie themed learning -gt

• Texas instruments has a program for modeling (via
  graphing) the spread of a Zombie Virus
• http//education.ti.com/en/us/stem-hollywood/zombi
  es
• Bio-Rads Secrets of the Rainforest Kit can be
  used in a Zombie apocalypse story where students
  use column chromatography to isolate the Zombie
  Green Fluorescent Protein.
• https//www.dropbox.com/sh/zbjkffhfsmmpurw/AAAifY6
  D85YqRwWoBEGe__LGa?dl0