What Nglycosylation steps would be good candidates for regulating Nglycan content and composition

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What N-glycosylation steps would be good
candidates for regulating N-glycan content and
composition? What are the functions of the
various subunits of the oligosaccharyltransfer
ase?  Consider the topology of N-glycosylation
and provide possible explanations for its
complexity.  Why is N-linked glycosylation more
likely to play a role than O-linked
glycosylation in protein folding? Compare the
processing of N-glycans in the ER and Golgi with
degradative pathways for N-glycans in lysosomes
and in the cytoplasm. How might diseases of
protein misfolding be treated therapeutically?
2
Need to control glycosylation site occupancy? How?
If N-glycosylation is so important, why only 1
set of genes? Loss causes lots of diseases
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Why use N-glycan addition for QC? Why not other
glycans?
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Why make this pathway so complicated?
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How to control of glycan structure? (Easy ways)
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What are the advantages of having
multi-antennary glycans?
How could you control composition of these
structures?
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What N-glycosylation steps would be good
candidates for regulating N-glycan content and
composition? What are the functions of the
various subunits of the oligosaccharyltransfer
ase?  Consider the topology of N-glycosylation
and provide possible explanations for its
complexity.  Why is N-linked glycosylation more
likely to play a role than O-linked
glycosylation in protein folding? Compare the
processing of N-glycans in the ER and Golgi with
degradative pathways for N-glycans in lysosomes
and in the cytoplasm. How might diseases of
protein misfolding be treated therapeutically?