Protein delivery: DNA nanostructures and cell-surface targeting - PowerPoint PPT Presentation

About This Presentation
Title:

Protein delivery: DNA nanostructures and cell-surface targeting

Description:

Can design DNA double helices to stick together and form interesting structures. A 1.7-kilobase single-stranded DNA ... c5.0 barrel (10 nM), 0.7% uranyl formate ... – PowerPoint PPT presentation

Number of Views:241
Avg rating:3.0/5.0
Slides: 21
Provided by: openwe
Category:

less

Transcript and Presenter's Notes

Title: Protein delivery: DNA nanostructures and cell-surface targeting


1
Protein delivery DNA nanostructures and
cell-surface targeting
Harvard iGEM August 27, 2006
2
The Machine
  • Goal Future modularized drug delivery

target cell
protein
3
DNA Nanostructures Overview
  • Can design DNA double helices to stick together
    and form interesting structures.
  • Dr. Ned Seeman, NYU
  • Dr. William Shih, Harvard
  • Paul Rothemund, Caltech

A 1.7-kilobase single-stranded DNA that folds
into a nanoscale octahedron WILLIAM M. SHIH, JOEL
D. QUISPE GERALD F. JOYCE?Nature 427, 618?621
(2004) doi10.1038/nature02307
4
Motivation Why DNA?
  • Fascinating area of research
  • The power of DNA
  • Watson-Crick base pairing is enormously strong
  • Self-assembly
  • Highly programmable, designable
  • Specificity - targeting to cells

5
Design Details
6
Design Details Scaffolded Oragami
7
Design Details Scaffolded Oragami
8
Design Details Scaffolded Oragami
9
Design Details Positional Control
10
Design Details Positional Control
11
Design Details Positional Control
12
Design Details Positional Control
13
Design Details Positional Control
14
Design Details Positional Control
15
Progress
  • Built a number of barrel designs
  • Exciting EM Images
  • Purifying Nanostructures (nearly there after 1
    month of trials)

16
Exciting EM Images
17
Exciting EM Images
18
EM Images (snakes on a grid)
  • c5.0 barrel (10 nM), 0.7 uranyl formate
  • Appear to be lining up end to end, probably
    because of the stain

Images courtesy Shawn Douglas
19
To be continued
protease
  • Can a protein be protected from protease if
    attached inside the box?
  • Lid attachment
  • Lid removal

protein
protease
protein
20
Acknowledgements
  • Harvard TFs - Shawn Douglas, Nick Stroustrup,
    Chris Doucette
  • Harvard advisers - Dr. William Shih, Dr. George
    Church, Dr. Pamela Silver, Dr. Alain Viel,
    Dr. Jagesh Shah, Dr. Radhika Nagpal
  • iGEM ambassadors
  • iGEM directors
Write a Comment
User Comments (0)
About PowerShow.com