HPLC Phytoplankton Pigment Measurements: Continuation of Climate Quality Data Records (CQDR) in Support of NASA

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HPLC Phytoplankton Pigment Measurements
Continuation of Climate Quality Data Records
(CQDR) in Support of NASAs Earth Science
Enterprise (ESE) C.C. Trees and J.L.
Mueller Center for Hydro-Optics and Remote
Sensing (CHORS) San Diego State
University (ctrees_at_chors.sdsu.edu)
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HPLC Phytoplankton Pigment Protocols (Bidigare et
al., 2003 NASA/TM-2003-211621/Rev 5 - Vol. V)

• MODIS Data Products Program 4,800 samples/yr
• Method Comparison
• Co-elution of mono- and divinyl Chl a
• 1. Wright et al. (1991) C18 Method (SIMBIOS)
• Channels ratio (436 450nm)
• Physical separation of mono- and divinyl chl a
• 1. Goericke and Repeta (1993) C8 Method
• 2. Van Heukelem and Thomas (2001) C8 with
• column heater Method

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Divinyl Monovinyl Chl a (664 nm)
Divinyl and Monovinyl Chl a Separation
Divinyl Chl a
Monovinyl Chl a
Divinyl Chl b
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Culture Mixture (450 nm)
Mixture of Pigments from Cultures
Fucoxanthin
Monovinyl Chl a
Chl c3
Injection Spike
Diadinoxanthin
19-Hexanoyloxyfucoxanthin
Monovinyl Chl b
Peridinin
Chl c2
b.b carotene
Zeaxanthin
Prasinoxanthin
Monovinyl Chls a, a
Alloxanthin
Diatoxanthin
b.e carotene
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HPLC Samples Processed/Submitted
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CHORS Pigment Database
Total Accessory 1.082 Total Chl a 0.906
R2 0.956
n 20,297