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Nucleotide Structure: Pentose sugar

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Nucleotide Structure: Pentose sugar Nucleotide Structure: Nitrogenous Bases DNA Linkage Phosphate group/phosphodiester bond Nucleotides contain one, two, or three ... – PowerPoint PPT presentation

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Title: Nucleotide Structure: Pentose sugar


1
Nucleotide Structure Pentose sugar
2
Nucleotide Structure Nitrogenous Bases
3
DNA Linkage
  • Phosphate group/phosphodiester bond
  • Nucleotides contain one, two, or three phosphate
    groups

nucleoside nucleotide
4
Nucleic Acid Polarity
  • Top of molecule has
  • free 5-phosphate group
  • This is the 5 end
  • Bottom has a free 3
  • hydroxyl group
  • This is the 3 end

5
Structural analysis of DNA
Early 1950s Rosalind Franklin
1953 Watson and Crick
6
Complementarity
7
DNA Structure
8
(No Transcript)
9
Brief Review of DNA Replication
10
Nearest Neighbor Analysis (Kornberg 1956)
In vitro DNA synthesis Template DNA DNA
polymerase dNTPs
dTTP
dCTP
dGTP
dATP
Allow DNA replication to take place Denature
DNA Treat with micrococcal nuclease- yields
mononucleotides with 3 terminal phosphates
11
ssDNA

12
Exercise
List all possible dinucleotides (analysis of
nearest 5 neighbor)
Which must be present in equal frequencies if the
DNA strands are Parallel ? Antiparallel?
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