Morphology of microglial cells in wildtype mice and CD39 and CD73 mice

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Fundamental concept underlying this Action MS
is considered to be primarily a neuroinflammatory
disease AD is considered to be primarily a
neurodegenerative disease Both diseases show
both inflammatory and degenerative aspects We
feel that that each can learn from the other
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Objectives - Novel reagents, experimental
models, protocols, new applications of
existing models Focused projects that bridge
degeneration and inflammation research Integrated
cross-trained cadre of researchers
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• Key statistics
• At time of CSO Approval 20-11-2006
• 9 EU countries 1 non-COST country
• Now -
• 15 EU countries 2 non-COST 1 non
  COST-application
• First MC meeting 05-06-2007
• 1 Core group MCWG meeting in 2007, 2 planned
  for 2008
• 1 STSM approved and completed in 2007
• 1 STSM applied in 2007, approved and underway in
  2008
• User-friendly STSM guide on website

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Structure Chair / Secretary Trevor Owens,
Denmark (Stine Sonne Carstensen) Vice-chair Franc
esca Aloisi, Italy 4 Working groups WG1
Triggers Hugh Perry (UK), Francesca Aloisi
(Italy) WG2 Interactions Burkhard Becher
(Switzerland) Spyros Georgopoulos
(Greece) WG3 CNS response Bente Finsen (Denmark)
Knut Biber (Netherlands) WG4 Protection,Repair Alo
n Monsonego (Israel) Hans Lassmann
(Austria) STSM committee Jon Laman Netherlands
(Chair) Lesley Probert Greece Hugh Perry UK Alon
Monsonego Israel Training Joana Palha
Portugal Ingo Bechmann Germany
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Website http//www1.sdu.dk/multi/neurinfnet/ Open
ed May 2007 Hit counter installed 04-02-2008 Over
100 hits since then Downloads available Minutes
of MC meetings WG meeting programs, WG
reports COST guidelines (via link to COST
website) Neurinfnet in-house guidelines
(STSM) Password-protected area for data-sharing,
protocols Links COST website Individual
homepages, email contacts
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• Scientific progress in Year 1
• Core meeting of all WGs Zürich November 2007
• Focus on infection as a trigger (MCWG meetings
  Rome May 2008, Southampton November 2008)
• Genetic models for selective depletion of glial
  cells and pathways in models of MS and AD (STSM)
• Relative role of microglia vs. astroglia in MS
  and AD
• Stem cells - potential benefits and drawbacks
• The effect of migrating BM-derived cells in CNS
  disease
• Chronic glial activation impact on NPC
  differentiation and survival in hippocampus.
• Immune regulation and neuronal repair properties
  of NPC
• The effect of cytokines on axonal outgrowth
• Mission statement to be published as a jointly
  co-authored paper in 2008.

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• Challenges
• Neuropathology of MS versus AD
• Animal models
• Controversies
• Involvement of young scientists
• Publications
• Budgets and COST procedures

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COST-STSM-BM0603-2449Morphology of microglial
cells in wildtype mice versus CD39-/- and CD73-/-
mice
Ph.D. Student, Larisa Bulavina, Max Delbrück
Center for Molecular Medicine, Berlin Buch Host
Dr. B. Finsen, Medical Biotechnology Center,
University of Southern Denmark

• AIM To compare morphological parameters
• of microglial activation in the dentate gyrus
• Estimates of microglia
• - Numbers and density
• - Process length
• Stereological principles of sampling and
  counting
• - Largest and smallest somal diameter
• - Proximal process diameter
• Conventional morphometry
• - Volume of dentate molecular layer
• Cavalieri volume estimator

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Future plans Acquired and imposed immunity in
Alzheimers disease. Southampton November 17/18
2008 Organizers Hugh Perry, James Nicoll
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Future plans Acquired and imposed immunity in
Alzheimers disease. Southampton November 17/18
2008 Organizers Hugh Perry, James
Nicoll Training school The Life and Health
Sciences Institute, School of Health Sciences,
University of Minho, Portugal The aim of this
training school is to provide the basic language
on neuroscience and immunology for the attendees
to more easily profit from and provide input to
the various research lines gathered together
through this COST action. Spring 2009 Organizer
Joana Palha (with Euron, FENS)
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Future plans Acquired and imposed immunity in
Alzheimers disease. Southampton November 17/18
2008 Organizers Hugh Perry, James
Nicoll Training school The Life and Health
Sciences Institute, School of Health Sciences,
University of Minho Portugal The aim of this
training school is to provide the basic language
on neuroscience and immunology for the attendees
to more easily profit from and provide input to
the various research lines gathered together
through this COST action. Spring 2009 Organizer
Joana Palha (with Euron, FENS) Neuropathology
hands-on workshop, rotating to different
centers. 2008/2009 Frankfurt, Organizer Ingo
Bechmann
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