Teaching Methods for Apoptosis Using Various Disease Models

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Teaching Methods for Apoptosis Using Various
Disease Models

• Lia Amini, Ben Carr, Sarah Medearis, Beth
  Migliaccio, Mastafa Springston, Erica
  Sickelbaugh, Joe Jackson, Mandy Womble, Beth
  Hall, and Debbie Smith
• Faculty Advisor Dr. Clay Clark

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Overview of Apoptosis

• Lia Amini
• Ben Carr
• Sarah Medearis

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Apoptosis

• Programmed cell death
• Applications
• Proper development
• Tadpole tail
• Destruction of damaged cells
• Cancer
• Mechanism
• Initiation
• Integration and Control
• Execution
• Phagocytosis

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Extrinsic Pathway

• Signal binds to Membrane Receptors
• TNF-a
• Fas
• TRAIL
• Receptors migrate, co-aggregate
• This brings together DISC components inside the
  cell
• DISC activates capase-8, -10 this is
  facilitated by FADD and TRADD
• Caspase-8, -10 activate effector caspases
  caspase-3, -6, and 7
• Caspase-8 can also cleave Bid, which triggers
  mitochondrial damage. This essentially turns on
  the intrinsic pathway as a result of extrinsic
  pathway initiation.

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Intrinsic Pathway

• Mitochondria mediated
• Caused by stress factors
• Depletion of growth factors
• DNA damage
• Misfolded protein
• Under mitochondrial stress
• Mitochondrial outer membrane becomes permeable
• Reactive oxygen species production increases
• Fission of the mitochondria occurs

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Teaching Apoptosis in Primary Education

• Developed a coloring book and lesson plan to
  teach apoptosis
• Main goals
• What is apoptosis?
• Why does it happen?
• What are the two ways apoptosis is initiated?