CYTOLOGY - PowerPoint PPT Presentation

Loading...

PPT – CYTOLOGY PowerPoint presentation | free to download - id: 82d629-NTdiO



Loading


The Adobe Flash plugin is needed to view this content

Get the plugin now

View by Category
About This Presentation
Title:

CYTOLOGY

Description:

CYTOLOGY BY Dr. TAREK ATIA Histology and Cell Biology – PowerPoint PPT presentation

Number of Views:94
Avg rating:3.0/5.0
Slides: 23
Provided by: d668
Category:

less

Write a Comment
User Comments (0)
Transcript and Presenter's Notes

Title: CYTOLOGY


1
CYTOLOGY
CYTOLOGY
BY Dr. TAREK ATIA Histology and Cell Biology
2
BOOK
  • Color Textbook of
  • Histology
  • Leslie P Gartner
  • James L Hiatt

3
  • - Micro-techniques for tissue preparation.
  • - Stains.
  • - Microscopes.
  • - Special techniques used for medical diagnosis.
  • - Cell, Tissues and Organs.

4
Micro-techniques for tissue preparation
  • - Using Light or Electron Microscope.
  • 1- Fixation-
  • - Buffered formol saline
  • - 10 formalin
  • - Suza, Bouin, Zenker solution
  • - Formaldehyde or Glutradhyde
  • - Osmium tetraoxide
  • - Potassium permanganate

5
  • 2- Dehydration Gradual removal of water
    from the tissue using ascending grads of
    ethyl alcohol to prevent tissue shrinking.
  • 3- Clearing Replacement of alcohol in tissue by
    clearing fluid like xylen, benzene, or acetone

6
  • 4- Embedding
  • - Tissues are impregnated in paraffin
  • - Tissues are impregnated in Epon in
    gelatin capsule.
  • 5- Cutting
  • - Paraffin block are cut by microtome
    using metal knife, into thin
    sections 6µ
  • - Capsules are cut by ultra-microtome,
    using glass or diamond knife,
    into ultra thin sections
    50-100nm.

7
  • 6- Mounting
  • - Sections spread on the hot plate and
    mounted on glass slides.
  • - Sections mounted on metal grids.
  • 7- Staining
  • - Variable stains are used for specific
    tissues.
  • - Stained by heavy metals like lead
    nitrate and uranyl acetate.

8
(No Transcript)
9
(No Transcript)
10
Stains
  • - Special dyes used to stain the histological
    sections and make them ready for
    microscope examination.
  • - Heamatoxylin and Eosin are most common
    used.

11
Types of stains
  • - Reaction of stain
  • - Acidic Eosin stain
  • - Basic Heamatoxylin
    stain
  • - Neutral Leishman stain
  • - Physical stain stain dissolve in tissue
    without any chemical reaction such as
    - SUDAN III for fatty tissues.

12
  • - Vital stain Staining living tissue inside the
    body. Trypan blue stain.
  • - Supravital stain Staining living tissues
    outside the body. Brilliant cresyl
    blue.
  • - Metachromatic stain Staining the tissues with
    a color different from the original color of
    stain. Toluidin blue staining of Mast
    cells.

13
  • - Polychromatic stain Staining the tissues with
    multiple colors in spite of using a single
    stain. Geimsa stain for blood.
  • - Orthocromatic stain Staining the tissues with
    the same color of the stain, such as HE.
  • - Histo-chemical stain Staining the different
    chemical components of the cell.
  • - Immuno-histo-chemical stain Localisation and
    staining specific proteins by the antigen
    antibody reaction.

14
MICROSCOPY
  • - Light microscope
  • - Phase contrast microscope
  • - Polarizing microscope
  • - Fluorescence microscope
  • - Electron microscope Scanning / Transmission
  • - Interference microscope
  • - Confocal microscope
  • - Inverted microscope

15
Light microscope
16
(No Transcript)
17
Light microscope
Electron microscope
18
(No Transcript)
19
Special techniques used for diagnosis
  • - Histchemistry
  • - Immunohistochemistry
  • - Tissue / Cell Culture
  • - Cell Fractionation
  • - Exfoliative Cytology
  • - Cytogenetics and Molecular Cytogenetics
  • - Bone Marrow smear / Blood film
  • - Fine needle aspiration / biopsy
  • - Flowcytometry - Autoradiography

20
Cell fractionation
21
Histology and Cytology
22
Course of Histology
- Cytology Cell, Nucleus, DNA, and
Cytogenetics. - Tissues 4 basic tissues
including Epithelium,
Connective (Cartilage, Bone, Blood),
Muscular, and Nervous tissues. - Body
systems and different Organs.
About PowerShow.com