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Red Cell Volume

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Red Cell Volume Pictorial Guide To The RCV Procedure – PowerPoint PPT presentation

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Title: Red Cell Volume


1
Red Cell Volume
  • Pictorial Guide To The RCV Procedure

2
Blood Volume Determination
  • The fundamentals of the dilution principle are
  • based on the following equation and hold true for
    all dilution type equations that are applied in
    vitro
  • V Q
  • C
  • V Volume Q Dose of the activity injected
  • C Sample of the activity extracted

3
Applications of formula
V Q/C
Hence, by adding a known quantity of activity to
an unknown volume of liquid and extracted a
sample from the unknown liquid you can determine
its volume. This formula is the basis for all
volume determinations in nuclear medicine.
4
Equipment used Cr-51 Tag
  • Identify materials for the tagging of the RBCs
  • ACD vial
  • Shielding for ACD vial
  • Ascorbic Acid
  • Cr-51
  • Shielding
  • Cups for Standard Prep
  • Tubes for collecting RBCs

5
Preparing the syringe
  • Wet a 20 cc syringe with 1 ml of ACD Solution.
  • Using a 19 gauge needle take the syringe and draw
    15 ml of whole blood from the patient.

6
Preparing the blood for the tag
  • After drawing 15 ml of whole blood from the
    patient slowly add it to the ACD vial

7
Starting the tag
  • Add 100 uCi of Cr-51 to the ACD vial

8
Mixing the contents
  • Mix the contents in the ACD vial after adding
    contents
  • Mix every 5 to 10 minutes after that for a total
    of 30 minutes

9
Ampule of ascorbic acid
  • Concentration of ascorbic acid is 1000 mg/2ml
  • Calculate a 50 mg dose

10
Reducing the Cr-51
  • After 30 minutes add 30 - 50 mg of ascorbic acid
  • Mix contents again
  • This reduces the Cr-51 and locks it into the RBCs

11
End Product - Labeled RBCs
  • After waiting 5 minutes 10 ml is withdrawn from
    the ACD vial
  • Labeled RBCs are then injected into the patient
  • Dose circulates for 10 to 20 minutes before
    withdrawing WB2

12
Preparation of Standards
  • While the labeled RBCs are circulating in the
    patient make your standard.
  • 99 ml of water is measured twice and added to two
    separate containers.

13
The Standards
  • 1 ml of WB is added to 99 ml of H2O
  • After collecting a 4 ml blood sample it is spun
    down and 1 ml of Plasma is drawn off and added to
    99 ml of H2O
  • 2 - 4 ml samples from each container is drawn up
    to a test tube and labeled WB1 and Pl1

14
Patients Whole Blood
  • Using a 19 gauge needle with a 20 ml syringe
    approximately 20 ml of whole blood is drawn from
    patient
  • 4 - 4ml purple top tubes are used to collect 4
    equal samples of whole blood.
  • Three of these tubes are spin down to collect 4
    ml of plasma for Pl2

15
What about the other tubes?
  • 1 - 4 ml tube of patients whole blood is
    collected for WB2
  • 1 - 4 ml blood sample is collected from ACD vial
    to determine Hct1 from ACD vial
  • 1 - 4 ml blood sample is collected from pts to
    determine Hct2

16
Time to count
  • Count bkg for 20 minutes
  • Count all tubes for 20 minutes
  • Take them to the formula

17
Now just plug in the counts
WB1 - Pl1(1 - Hct1)
x Hct2 x 1000
RCV in ml
WB2 - Pl2(1 - Hct2)
18
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