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SALMONELLA TYPHIMURIUM- GETTING TO THE BOTTOM OF IT

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SALMONELLA TYPHIMURIUM- GETTING TO THE BOTTOM OF IT G. Doran , N. DeLappe, C. O Hare, G. Corbett Feeney, M. Cormican National Salmonella Reference Laboratory, Galway – PowerPoint PPT presentation

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Title: SALMONELLA TYPHIMURIUM- GETTING TO THE BOTTOM OF IT


1
SALMONELLA TYPHIMURIUM- GETTING TO THE BOTTOM OF
IT
  • G. Doran , N. DeLappe,
  • C. O Hare, G. Corbett Feeney,
  • M. Cormican
  • National Salmonella Reference Laboratory, Galway

2
INTRODUCTION
  • S. TYPHIMURIUM COMMON IN EUROPE, USA
  • POULTRY, CATTLE PIGS
  • MANY FOODS
  • MULTIDRUG RESISTANT ISOLATES

3
INTRODUCTION -2
  • MULTI DRUG RESISTANT DT104
  • INCIDENCE INCREASING
  • ACSSuT CHROMOSOMALLY CARRIED
  • TRIMETHOPRIM, NALIDIXIC ACID RESISTANCE
  • ? SUSCEPTIBILITY TO CIPROFLOXACIN

4
TYPING METHODS
  • PHAGE TYPING
  • ANTIMICROBIAL SUSCEPTIBILITY
  • PLASMID PROFILING
  • PFGE

5
PRINCIPLES OF PFGE
CATTTTAC GTAAAATG
6
PRINCIPLES OF PFGE
CATGTTAC GTACAATG NO CUT
7
PULSENET
  • STANDARDISED PFGE
  • CDC USA
  • E. coli, Salmonella, Listeria, Shigella
  • CENTRAL DATABASE

8
PFGE
1 2 3 4 5 6 7 8 9
10 11 12 13 14 15
9
PLASMID PROFILE
Size (MDa)
1 2 3 4 5 C C C C ? 6 7 8 9 10
125?
26 ?
10 ?
2 ?
10
S. TYPHIMURIUM 2001
  • 303 ISOLATES
  • ANIMAL AND HUMAN ORIGIN
  • PHAGE TYPING (N 303)
  • ANTIMICROBIAL SUSCEPTIBILITY (N 303)
  • PGFE TYPING (N 163)
  • PLASMID PROFILING (N 36)

11
(No Transcript)
12
ANTIMICROBIAL SUSCEPTIBILITY
13
PLASMID PROFILING
  • 36 ISOLATES
  • MODIFIED METHOD OF KADO AND LIU
  • PLASMIDS 1.5 - 125 MDA
  • 60MDA PLASMID IN DT104, MDR DT120 AND DT12

14
MDR
PATTERN
PHAGE TYPE
A
DT104, DT104b, MDR DT12 120 88 MDR
B
C
32 ISOLATES FULLY SENS
D
E
F
DT12 DT120
G
H
15
PHAGE TYPE U310
  • EMERGED IN 2001
  • PIGS, HUMANS
  • 15 ISOLATES
  • MINOR DIFFERENCES ON PFGE

16
DISCUSSION
  • S. TYPHIMURIUM
  • HUMANS ANIMALS
  • MDR - INCREASED
  • DT104 DT104b
  • MAJORITY MDR, CLONAL

17
DISCUSSION - 2
  • DT104, DT104b
  • PFGE U302, RDNC
  • P11 MDR DT12, DT120

P3, P5, P7, P14 DT193 P18, P20 P23, P27
18
CONCLUSIONS
  • PHENOTYPIC GENOTYPIC METHODS NECESSARY
  • HIGH INCIDENCE OF MDR IN IRELAND
  • CONTINUOUS MONITORING OF S. TYPHIMURIUM NECESSARY

19
OTHER RESEARCH
  • FLUROQUINOLONE RESISTANCE IN S. ENTERITIDIS
  • DONNA KILMARTIN
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