Study%20of%20Influence%20of%20Salts%20Additives%20to%20Mixed%20Reverse%20Micelles%20via%20Reverse%20Microemulsion%20Chromatography%20and%20UV-visible%20spectroscopy

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Study of Influence of Salts Additives to Mixed
Reverse Micelles via Reverse Microemulsion
Chromatography and UV-visible spectroscopy

• Manuchar Gvaramia,
• Postgraduate student, Department of Chemistry,
  Faculty of Exact and Natural Sciences,
• Tbilisi University, Georgia

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Reverse micelle
Direct micelle
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Mixed microemulsions on the basis of nonionic and
anionic surfactants
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Dependence of retention factors of solutes on the
water/surfactant ratio
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Dependence of retention factors of solutes on the
water/surfactant ratio
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Water,Brij-30, butanol and hexane with ratio
(v/v.) 0.3/2.8/4.6/92.3
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Water,Brij-30, butanol and hexane with ratio
(v/v.) 0.7/2.8/4.6/91.9
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Diagram of retention factors of solutes at w2(
) and w3 ( ) in the presence of sodium
acetate(0.025M)
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Diagram of retention factors of solutes at w2(
) and w3 ( ) in the presence of sodium
acetate(0.025M)
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Diagram of retention factors of solutes at
w2() and w3() in the presence of potassium
perchlotate(0.025)

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Diagram of retention factors of solutes at
w2() and w3() in the presence of potassium
perchlotate(0.025)
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Diagram of retention factors of solutes at the
presense of sodium acetate () and potassium
perchlorate() W2
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O-nitroaniline as molecular probe
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Determination of binding constants of
o-nitroaniline (o-NA) to Brij-30 reverse micelles
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Absorption spectra of o-NA in hexane/(?-0.0M
Brij-30 0.1M AOT, 0.13 10-2M Brij-30 0.1AOT,
0.9110-2M Brij-30 0.1M AOT, 2.7310-2M
Brij-30 0.1M AOT, 11.710-2M Brij-300.1M AOT,
23.410-2M Brij-30 0.1M AOT) at Brij-30
concentrations. At W00, o-NA4.510-5M
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Variation of ?m of o-NA in reverse micelles as a
function of Brij-30 concentration
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Diagram of Kb of o-NA with (Brij-30AOT) mixed
micelles in the following conditions1) absence
of water, 2) presence of water, 3) presence of
0.05M sodium acetate and 4) 0.05M potassium
perchlorate
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a) Diagram of retention factors of solutes in the
presence of sodium acetate () and potassium
perchlorate (?) W2b) Diagram of binding
constants of o-NA with (Brij-30AOT) mixed
micelles in the presence of 0.05M sodium
acetate() and 0.05M potassium perchlorate(?)
b
a
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Conclusions

• Binding of o-nitroaniline to reverse micelles is
  higher in the presence of chaotropic perchlorate
  ions in comparison with kosmotropic acetate ions.
• Solubility of the model compounds is lower in the
  presence of chaotropic anions in the water pools
  of reverse micelles as compared with kosmotropic
  acetate ions.
• Retention factor of the solutes are higher in the
  presence of kosmotropic acetate ions in the water
  nanocages of the reverse micelles as compared to
  perchlorate ions.

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Conclusions

• Results may be useful in the investigations of
  water structure, when it is confined to
  nanometer-scale cavities, viz. in biological
  systems.

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Acknowlegement

• I thank the Georgian Shota Rustaveli National
  Science Foundation and Julich Scientific Center
  for opportunity to participate in this project

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Thanks for attention
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• SO42- gt F- gt OAc- gt Cl- gt Br- gt NO3- gt I- gt ClO4-
  gt SCN-

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Benzene-1,3-diol resorcinol
benzene-1,2,3-triol pyrogallol
benzene-1,4-diol hydroquinone
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1,3,7-trimethyl-1H-purine-2,6(3H,7H)-dione caffein
e
1,3-dimethyl-7H-purine-2,6-dione theophylline
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2,2,2-trichloroethane-1,1-diol
Phenobarbital (5-ethyl-5-phenylbarbituric acid or
5-ethyl-5-phenylhexahydropyrimindin-2,4,6-trione)

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carbamazepine
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Absorption spectra of o-NA in different
solvents1) water (412), 2) ethanol (406 nm), 3)
methanol (405nm) and 4)isopropanol (407 nm)
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Possible scheme of arrangement of ions in water
pools of the reverse micelle